P-GP、GST-π在妊娠性滋养细胞肿瘤中的表达及其与化疗耐药性的关系

The expressions of P GP and GST π in gestational trophoblastic tumor and their relationships with chemotherpeutr resistance.

目的：研究妊娠滋养细胞肿瘤（ＧＴＴ）中Ｐ糖蛋白（Ｐ－ＧＰ）和谷胱甘肽Ｓ转移酶（ＧＳＴ－π）的表达与化疗耐药性的关系。方法：应用免疫组化法检测Ｐ－ＧＰ和ＧＳＴ－π在ＧＴＴ中的表达。结果：正常早孕绒毛、完全性葡萄胎、侵蚀性葡萄胎及绒毛膜癌中的Ｐ－ＧＰ表达逐渐降低（Ｐ＜０．０５）。Ｐ－ＧＰ在病灶直径≥３ｃｍ和ＷＨＯ评分≥８分者的染色强度低于＜３ｃｍ及＜８分者。化疗耐药者Ｐ－ＧＰ表达与敏感者差异无显著性（Ｐ＞０．０５），耐药者ＧＳＴ－π表达明显高于敏感者（Ｐ＜０．０１）。结论：Ｐ－ＧＰ与ＧＴＴ分化程度及患者预后有关。ＧＳＴ－π为ＧＴＴ获得性耐药机制之一，对ＧＴＴ病例的耐药性评价有重要意义。

Objective:To study on relationships among the expressions of P glycoprotein(P GP) and Glutathione sulfur transferases π(GST π)and gestational trophoblastic tumor(GTT)with chemotherapy resistance.Methods:The expressions of P GP and GST π were measured by immunohistochemical technique.Results:The expression of P GP decreased gradually in the order of normal villous,hydatidiform mole,invasive hydatidiform mole and choriocarcinoma( P <0.005).The focus <3cm group and WHO scoring <8 group were stained significantly stronger than those in their counterparts( P <0.05).The expression of P GP had no significant difference between chemotherapeutic resistant group and sensitive group( P >0.05).The expression of GST π in resistant group was stronger than that of sensitive group( P <0.01).Conclusions:P GP has a relationship with the differentiation and may serve as a indictor to predict the prognoses of patients with GTT.GST π participates in mechanisms of acquried drug resistance and it has an important significance of immunohistochemistry to evaluate the chemotherapeutic resistance in GTT.