妊高征患者外周血LGLs和TNF-α水平的测定

Determination of large granular lymphocytes and tumor necrosis factor α in peripheral plasma of pregnancy induced hypertersion syndrome.

目的：探讨大颗粒淋巴细胞（ＬＧＬｓ）和肿瘤坏死因子－α（ＴＮＦ－α）与妊高征发病的关系。方法：应用双抗体夹心酶联免疫吸附法（ＥＬＩＳＡ）、全血细胞分析仪结合油镜下计数ＬＧＬｓ占淋巴细胞的百分数检测妊高征３９例（妊高征组），正常孕晚期４０例（正常妊娠组）和健康育龄非妊娠４０例（对照组）外周血ＴＮＦ－α含量和ＬＧＬｓ数量。结果：妊高征组外周血ＬＧＬｓ和ＴＮＦ－α水平高于正常妊娠组、对照组，以中、重度妊高征患者增高最为显著（Ｐ＜０．０５），其中合并胎儿宫内生长迟缓（ＩＵＧＲ）者外周血ＬＧＬｓ和ＴＮＦ－α水平与未合并ＩＵＧＲ者差异无显著性（Ｐ＞０．０５）。结论：细胞免疫，特别是ＬＧＬｓ和ＴＮＦ－α参与了妊高征的病理机制。

Objective:To determine the quantity of large granular lymphocytes (LGLs) and tumor necrosis factor α(TNF α) concentration in peripheral plasma of pregnaney induced hypertension syndrome (PIHS).Methods:Forty cases of normal term pregnant women(NP group),thirty nine cases of PIHS (PIHS group)and forty cases of healthy childbearing age nonpregnant women (control group) were measured.LGLs was quantified by counting under microscope with oil immersion lens(100×10)and blood cells were categorized by bioassay.TNF α was measured with a sensitive double antibody sandwich enzyme ELISA.Results:Peripheral plasma LGLs and TNF α levels in PIHS group were generally higher than those in NP group and control group,especially higher levels in the moderate and severe PIHS( P <0.05).The levels of plasma TNF α and LGLs were not significantly different between those in PIHS without IUGR.Conclusion:Cell mediated immunity,especially LGLs and TNF α participates in the pathomechanism of PIHS.