牛心朴子生物碱对奶牛乳房炎病原菌的体外抑菌试验

In Vitro Bacteriostasis of Cynanchum Komarovii against Mastitis Pathogens in Dairy Cows

对4例临床型奶牛乳房炎的乳汁进行病原分离及鉴定,分离出了金黄色葡萄球菌、无乳链球菌和大肠杆菌。选择牛心朴子生物碱,采用试管2倍稀释法进行体外抑菌试验,测其最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果显示:牛心朴子生物碱对3种菌都有不同程度的抑制作用,对金黄色葡萄球菌、无乳链球菌、大肠杆菌分离株的MIC及MBC分别是12.5mg/mL、1.561 25mg/mL、6.25mg/mL和6.25mg/mL、1.561 25mg/mL、3.125mg/mL。

StaphyLococcus aureus, Streptococcus agalactiae, and Escherichia coli were isolated from 4 milk samples of dairy cows with clinical mastitis respectively. Cynanchum Komarovii alkaloid was chosen, test tube 2-fold dilution method was used to conduct inhibitory test on 3 pathogens and determined the minimal inhibitory concentration （MIC）and minimum bactericidal concentration （MBC）. The resuk showed that the alkaloid had different degree bactenostasis on StaphyLococcus aureus, Streptococcus agalactiae, and Escherichia coli. The MIC and MBC to the above three tested bacteria were 12.5mg/mL, 1.561 25mg/mL, 6.25mg/mL and 6.25mg/mL, 1.561 25mg/mL, 3.125mg/mL respectively.