期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

肾癌OS-RC-2细胞CD133的表达及意义 被引量：1

Expression and significance of CD33 in renal carcinoma stem cells line OS-RC-2
下载PDF
导出
摘要 目的:培养肾癌干细胞球,研究其表面分子特性。方法:肾癌细胞悬浮于含有表皮生长因子(epidermal growth factor,EGF)和成纤维生长因子(basic fibroblast growth factor,bFGF)的无血清培养基中培养,培养7天后收集细胞球,流式细胞仪检测CD133及CD34的表达。结果:悬浮培养2天后出现肾癌干细胞球,培养7天后干细胞球明显增多,流式细胞仪检测发现表达CD133+CD34-细胞约占(8.33±1.26)%,对照肾癌细胞组表达CD133+CD34-细胞只占(1.24±0.36)%;干细胞球通过含10%胎牛血清培养基继续培养,又恢复原来的生长特点。结论:利用无血清悬浮培养方法得到的肾癌干细胞球高表达干细胞表面分子标记CD133。 Objective： To cultivate renal cell carcinoma（RCC） stem cells in serum free medium（SFM） with the method of suspension culture and to research their property of surface molecules on the cells.Methods： RCC cells were collected and dissociated in to single ones,then they were seeded in SFM containing EGF and bFGF.Seven days later the expression of CD133 and CD34 in cells from SFM was detected by flow cytometry. Results： RCC stem cells spheres were appeared after RCC cells had been cultured two days in SFM;Seven days later, more spheres came out . Then collected and detected them by flow cytometry.We found that about （ 1.24 ± 0.36） % CD133 ^＋ CD34^- cells were in normal OS-RC-2 cell line, while it reached to （8.33 ± 1.26） % after cultivating in SFM for 7 days. After the cell spheres were recuhivated in SSM, CD133 ^＋ CD34^-cells became less and close to before. Conclusion： RCC stem cells spheres enriched from RCC cells by SFM had higher expression of CD133.
作者 田福起 孙浩 潘鹏 郭涛
机构地区 江苏大学附属医院泌尿外科
出处 《江苏大学学报（医学版）》 CAS 2009年第5期395-397,共3页 Journal of Jiangsu University：Medicine Edition
基金 镇江市科技基金资助项目(SH2007024)
关键词 肿瘤干细胞 肾癌 细胞培养 CD133 CD34 tumor stem cell renal cell carcinoma（RCC） cell culture CD133 CD34
分类号 R737.11 [医药卫生—肿瘤]
  • 相关文献

参考文献9

  • 1Patrik CW,Alan BR. Campbell's urology [ M]. 7th ed. Volume 3. Beijing: Science Press, 2001:2292 - 2293.
  • 2吴阶平,顾方六,郭应禄,等.吴阶平泌尿外科学[M].山东:山东科学技术出版社,2004:965-966.
  • 3Siapati EK, Bigger BW, Kashofer K, et al. Murine leukemia following irradiation conditioning for transplantation of lentivirally-modified hematopoietic stem cells [ J ]. Eur J Haematol,2007,78 ( 4 ) : 303 - 313.
  • 4Stupp R, Hegi ME. Targeting brain-tumor stem cells [ J ]. Nat Biotechnol,2007,25 (2) : 193 - 194.
  • 5Singh SK, Dirks PB. Brain tumor stem cells: identification and concepts[J], Neurosurg Clin N Am,2007, 18 (1) :31 -38.
  • 6Calabrese C,Poppleton H, Kocak M, et al. A perivascular niche for brain tumor stem cells[J]. Cancer Cell, 2007,11 (1):69 -82.
  • 7Miraglia S, Godfrey W, Yin AH, et al. A novel five-transmembrance hematopoietic stem cell antigen: isolation, characterization, and molecular cloning [ J ]. Blood, 1997, 90(12) :5013 -5021.
  • 8Bussolati B, Bruno S, Grange C, et al. Isolation of renal progenitor cells from adult human kidney [ J ]. Am J Pathol, 2005,166 ( 2 ) : 545 - 555 .
  • 9翟羽佳,常文军,侯建国,谭晓洁,马立业,曹广文.肾透明细胞癌CD133的表达及其体外对α-干扰素和5-氟尿嘧啶处理效果的影响[J].第二军医大学学报,2009,30(3):252-255. 被引量：6

二级参考文献15

  • 1邓永文,方加胜,李茗初,陈风华,刘劲芳,伍军,周向阳,方芳,卢明,陈成,周仁辉,曾飞跃.胶质瘤中肿瘤干细胞的分离、培养及鉴定[J].中国现代医学杂志,2005,15(16):2449-2452. 被引量：16
  • 2方加胜,邓永文,李茗初,陈风华,王延金,卢明,方芳,伍军,杨转移,周向阳,王飞,陈诚.神经上皮来源脑肿瘤中肿瘤干细胞的分离培养与鉴定[J].中华医学杂志,2007,87(5):298-303. 被引量：10
  • 3Singh S K,Clarke I D, Terasaki M, Bonn V E, Hawkins C, Squire J, et al. Identification of a cancer stem ceil in human brain tumors[J]. Cancer Res, 2003,63 : 5821-5828.
  • 4Donnenberg V S,Donnenberg A D. Multiple drug resistance in cancer revisited: the cancer stem cell hypothesis[J]. J Clin Pharmacol, 2005,45 : 872-877.
  • 5Dean M, Fojo T, Bates S. Tumour stem cells and drug resistance[J]. Nat Rev Cancer,2005,5:275-284.
  • 6Kondo T,Setoguchi T,Taga T. Persistence of a small subpopulation of cancer stem-like cells in the C6 glioma cell line[J]. Proc Natl Acad Sci USA, 2004,101: 781-786.
  • 7Al-Hajj M, Wicha M S, Benito-Hernandez A, Morrison S J, Clarke M F. Prospective identification of tumorigenic breast cancer cells[J]. Proc Natl Acad Sci USA, 2003,100 : 3983-3988.
  • 8Kim C F,Jackson E L, Woolfenden A E, Lawrence S, Babar I, Vogel S, et al. Identification of bronchioalveolar stem cells in normal lung and lung cancer[J]. Cell,2005,121:823-835.
  • 9Collins A T, Berry P A, Hyde C, Stower M J, Maitland N J. Prospective identification of tumorigenic prostate cancer stem cells[J]. Cancer Res, 2005,65 : 10946-10951.
  • 10Li C,Heidt D G,Dalerba P,Burant C F,Zhang L,Adsay V,et al. Identification of pancreatic cancer stem cells [J]. Cancer Res, 2007,67 : 1030-1037.

共引文献58

同被引文献11

  • 1Singh SK,Clarke ID,Terasaki M,et a1.Identification of a cancer stem cell in human brain tumors[J].Cancer Res,2003,63(18):5821-5828.
  • 2Palm T,Schwamborn JC.Brain tumor stem cells[J].Biol Chem,2010,391(6):607-617.
  • 3Mathieu J,Zhang Z,Zhou W,et al.HIF induces human embryonic stem cell markers in cancer cells[J].Cancer Res,2011,71(13):4640-4652.
  • 4Yin AH,Miraglia S,Zanjani ED,et al.AC133,a novel marker for human hematopoietic stem and progenitor cells[J].Blood,1997,90(12):5002-5012.
  • 5Chen KH,Hsu CC,Song WS,et al.Celecoxib enhances radiosensitivity in medulloblastoma-derived CD133-positive cells[J].Childs Nerv Syst,2010,26(11):1605-1612.
  • 6Jin F,Gao C,Zhao L,et al.Using CD133 positive U251 glioblastoma stem cells to establish nude mice model of transplanted tumor[J].Brain Res,2011,1368(1):82-90.
  • 7Singh SK,Hawkins C,Clarke ID,et al.Identification of human brain tumour initiating cells[J].Nature,2004,432(11):396-401.
  • 8Zeppernick F,Ahmadi R,Campos B,et al.Stem cell marker CD133 affects clinical outcome in glioma patients[J].Clin Cancer Res,2008,14(1):123-129.
  • 9Joo KM,Kim SY,Jin X,et al.Clinical and biological implications of CD133-positive and CD133-negative cells in glioblastomas[J].Lab Invest,2008,88(6):808-815.
  • 10Liao Y,Hu X,Huang X,et al.Quantitative analyses of CD133 expression facilitate researches on tumor stem cells[J].Biol Pharm Bull,2010,33(5):738-742.

引证文献1

二级引证文献1

江苏大学学报（医学版）
2009年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部