创伤后T细胞功能与抑制性T细胞及反抑制T细胞活性变化的关系

Relationship between T Cell function and changes in suppressor T cells and contrasuppressor T cells activities after trauma

目的：研究创伤后Ｔ细胞功能与抑制性Ｔ细胞（Ｔｓ）及反抑制Ｔ细胞（Ｔｃｓ）活性变化的关系。方法：利用小鼠截肢伤模型，观察Ｔ细胞功能的变化，并测定Ｔｓ细胞对正常Ｔ细胞功能的抑制活性以及Ｔｃｓ细胞对Ｔｓ细胞的反抑制活性变化。结果：创伤小鼠脾细胞的Ｔ淋巴细胞转化活性降低，辅助性Ｔ细胞（Ｔｈ）、Ｔｃｓ细胞数目一过性减少，Ｔｓ细胞数目一过性增多；创伤后Ｔｓ细胞对正常Ｔ淋巴细胞转化、白细胞介素２（ＩＬ２）生成、ＩＬ２受体α（ＩＬ２Ｒα）表达、ＩＬ２ｍＲＮＡ及ＩＬ２ＲαｍＲＮＡ水平的抑制作用明显增强，而Ｔｃｓ细胞对Ｔｓ细胞的反抑制活性明显减弱；Ｔｓ细胞抑制率及Ｔｃｓ细胞反抑制率变化与Ｔ淋巴细胞转化活性降低均密切相关；去除创伤小鼠脾细胞中Ｔｓ细胞，则可明显提高Ｔ淋巴细胞转化活性、ＩＬ２生成及ＩＬ２Ｒα表达；正常Ｔｃｓ细胞对伤后Ｔｓ细胞抑制作用具有一定的逆转效应。结论：创伤后Ｔｓ细胞活性增强。

Objective:To investigate the relationship between T cell function and changes in suppressor T cells (Ts),contrasuppressor T cells (Tcs) activities after trauma.Methods:Using a murine amputation injury model,changes in T cell function were observed,and suppressive response of Ts on normal T cell function and contrasuppressive effect of Tcs were measured.Results:T lymphocyte transformation of splenocytes was reduced,the numbers of helper T cells (Th) and Tcs were transiently decreased,while that of Ts was increased in traumatized mice.Following trauma,suppressive responses of Ts on normal T lymphocyte transformation,interleukin2 (IL2) production,IL2 receptorα (IL2Rα) expression,IL2 mRNA and IL2Rα mRNA levels were significantly enhanced,however,contrasuppressive effect of Tcs on Ts were obviously alleviated.The changes in suppressive rate of Ts and contrasuppressive rate of Tcs were closely related to decreased T lymphocyte transformation after trauma.Removing Ts from splenocytes could significantly increase T lymphocyte transformation,IL2 production and IL2Rα expression.Normal Tcs showed reverse effect to a certain extent on suppressive activity of Ts posttrauma.Conclusions:Enhanced Ts activity and reduced Tcs activity after trauma may participate in mediating depression of T cell function.