细胞色素P450 2E1(CYP2E1)的遗传多态性与非吸烟女性肺癌的危险度

CYTOCHROME P450 2E1 GENETIC POLYMORPHISM AND NON-SMOKING FEMALE LUNG CANCER RISK

我们在上海和哈尔滨二地分别收集了非吸烟女性肺癌病例各１００例和８２例以及非吸烟的女性健康人对照各９５例和８９例。分析了白细胞ＤＮＡ的细胞色素Ｐ４５０２Ｅ１基因第六内含子ＤｒａＩ切点和５’端调控区ＲｓａＩ切点的遗传多态性。虽然上海病例组ＤｒａＩ和ＲｓａＩ突变型纯合子与对照组比ＯＲ值分别为２５６和５４７，但未达显著性；哈尔滨病例组ＯＲ值分别为０４９和０４７，也无显著差异。

The blood of 100 non-smoking female lung cancer cases and 95 healthy cantrols,82cases and 89 controls were collected from Shanghai and Harbin population respectively. The DNA were extracted from buffy coat with phenol chloroform. Cytochrome P450(CYP)2E1 genotype were analyzed with restriction enzyme DraI for the sixth intron and Rsa I for the 5' flanking region. The OR reach 2.56 and 5.47 for CYP2E1 Dra I and Rsa I homozygous mutant respectively in Shanghai cases, but there were no statistic significance. Though in Harbin cases the OR reach 0.49 and 0.47, also no significance.