紫果西番莲染色体制片方法比较及其核型分析

Comparison of chromosome spreading methods and karyotype of Passiflora edulis Sims

比较了不同预处理方法、解离方法、酶解时间和制片方法对紫果西番莲细胞中期分裂相、染色体收缩程度和分散度的影响。结果表明:8-羟基喹啉室温下处理根尖2.5 h、混合酶液(2%纤维素酶和0.5%果胶酶)37℃酶解70 min、涂片法制片的染色体分散性好,背景干净,着丝点比较清晰,随体明显,效果最佳;紫果西番莲的核型公式是2n=2x=18=14m(2sat)+4sm,属于2A型,第4对染色体上带有随体。

Effects of different pretreatments methods,dissociation,enzymolysis time and slide making on the metaphase cells,chromosome contraction and dispersion degree of P.edulis Sims were compared.The best metaphase cells were prepared after the pretreatment with 8-Hydroxyquinoline for 2.5 h at room temperature and the subsequent enzyme digestion by a mixture containing 2% cellulase and 0.5% pectinase for 70 min at 37 ℃.The chromosomes dispersed well with clean background and centromeric dots and satellite were clear.The karyotype formula of P. edulis Sims was 2n=2x= 18=14m （2sat） ＋4sm, belonging to 2A type, and a pair of satellites were located on the forth pair chromosomes.