釉原蛋白在小鼠牙胚发育中的时空表达

Spatio-temporal expression of amelogenin during odontogenesis in mus musculus

目的:探讨釉原蛋白(Amelogenin)在小鼠牙胚发育表达中的时空顺序、分布及牙齿发育矿化中的作用。方法:采用原位杂交的实验方法,观察釉原蛋白在牙胚发育期的增殖分化期、分泌初期及分泌期的表达。结果:釉原蛋白mRNA在成釉细胞增殖分化期表达为阴性;分泌初期细胞内及新生釉基质中开始出现阳性表达;至分泌期,细胞内及新生釉基质中均呈强阳性表达。成熟釉质中釉原蛋白mRNA无表达。在釉牙本质界处可检测到釉原蛋白mRNA的弱阳性表达。结论:本实验从分子水平上进一步明确了釉原蛋白的时空表达,并推测釉原蛋白不仅介导了釉质的发育矿化,与牙齿发育、釉基质矿化反应等过程密切相关,还可能对成牙本质细胞有着分化诱导的作用。

Objective：To study the expression of amelogenin in different development stages of Mus musculus tooth germ, and to discuss the function of amelogenin in the development of enamel.Methods：We performed in situ hybridization after preparing specimens of different stages of the incisor developing tooth germ in Mus musculus.Results：Amelogenin mRNA was negative under microscope in the ameloblast proliferate differentiation period, it showed weakly positive expression in intra-cellular and new-birth enamel matrix in secretory initial stage while it showed strongly positive expression in intra-cellular and new-birth enamel matrix when it came to secretion phase.There was no positive expression of ameloblastin mRNA in mature stage. Amelogenin mRNA was detected to be weakly positive near the dentoenamel junction.Conclusion：This experiment clarifies the spatio-temporal expression of amelogenin from the molecular level, and predicts that amelogenin not only mediates the growth and mineralization of enamel,but also closely relates to the process of the growth of teeth and mineralizing reaction of enamel matrix as well. Furthermore, amelogenin also play a role in differentiation and induction to odontoblast.