菠萝皮渣半纤维素多糖提取纯化及性质研究

Extraction,Purification and Properties Researches of Hemi-cellulose Polysaccharides from Pineapple Peel

通过正交试验优化了碱提醇沉菠萝皮渣半纤维素粗多糖提取条件,并用DEAE-Cellulose(OH-)纯化得到3种半纤维素多糖组分,初步分析了半纤维素多糖的理化性质。结果表明:半纤维素粗多糖最佳提取条件为时间2 h,NaOH质量分数7%,固液比(g∶mL)1∶10,温度40℃;菠萝粗半纤维素得率和聚戊糖含量分别为41.3%和73%;柱层析菠萝半纤维素多糖-1和2基本不含单糖、淀粉、蛋白质、核酸类物质;红外分析表明菠萝半纤维素多糖-2具有典型多糖吸收图谱。

The pineapple tion and ethanol precipitation, hemi-cellulose polysaccharides, obtained from pineapple peel by NaOH solution extracwere separated and then purified by DEAE-Cellulose（OH-）column. The optimal processing of pineapple hemi-cellulose polysaccharides extraction was： extracting time of 2h, ratio of material and 7% NaOH （w： v） 1： 10, extracting temperature of 40℃. The yield of hemi-cellulose and content of pentosans are 41.3% and 73% respectively. Three kinds of purified hemi-cellulose polysaccharides by freeze-drying were obtained. The he- mi-cellulose polysaccharides-1,2 were basically not composed of monosaccharide, starch, protein and nucleic acid. The hemi-cellulose polysaccharides-2 was showed a typical polysaccharide FT-IR spectrum profile.