超声与双水相体系耦合提取芒果核多酚及活性研究

Study on the Ultrasound Extraction Coupling with Two-phase Aqueous System in the Extraction of Polyphenols from Mango Nuclear and Its Antioxidantion Activity

利用超声与丙醇-硫酸铵双水相体系耦合对芒果核多酚进行提取分离,通过正交试验法对双水相形成和超声提取条件进行优化,最佳提取条件是:丙醇体积分数60%,超声提取15 min,(NH4)2SO4用量为3 g/L,料液比(g∶mL)为1∶20,在此提取条件下多酚率提取率为6.96%,提取物中植物多酚纯度为37.8%,明显高于回流提取法。提取物对脂质过氧化及诱导红细胞氧化损伤具有良好的抑制作用,最大抑制率分别为66.8%和67.4%,方法简便、条件温和,环境友好。

The ultrasound extraction technology coupling with propyl-alcohol ammonium sulfate aqueous two- phase separation for extraction of polyphenols from mango nuclear was studied. The formation of aqueous two-phase and ultrasonic extraction conditions were optimized by orthogonal test, the optimum extraction conditions were： the propyl-alcohol solution （ volume fraction as 60 % ） , extraction time 15 min, 0.30 （ NH4） 2SO4 0.30 g/mL, the ratio of materials to solution as 1 ： 20 （ g/mL）. The extraction rates of polyphenols was 6.96 % （ W/W）, the purity of polyphenols was 37.8 % （W/W） which was much higher than that of refluent ethanol extraction. The extractives singificantly inhibit the lipid peroxidation （LPO） induced by ·OH free radical and hemoglobin （RBC）oxidate injury induced by free radical with the maximum inhibition rates of 66.8 % and 67.4 % , respectively. The proposed method is simply, mildly conditions and environmentally friend.