期刊文献+

北京地区健康成人外周血T淋巴细胞亚群的参考范围 被引量:14

下载PDF
导出
摘要 目的建立北京地区健康成人外周血T淋巴细胞亚群参考范围。方法采用多参数三色免疫荧光标记流式细胞术结合血液分析仪检测403例北京地区健康成人的外周血CD4+及CD8+T淋巴细胞亚群。结果外周血T淋巴细胞亚群在各年龄组间无显著性差异。男、女间外周血白细胞计数、淋巴细胞绝对值、CD3+细胞百分率、CD3+CD4+细胞绝对值、CD3+CD8+细胞绝对值有显著性差异。北京地区健康成人外周血CD4+及CD8+T淋巴细胞参考范围(以95%置信区间表示)如下:淋巴细胞1210~3011cell/μl(22.4%~46.6%);CD3+细胞808~2072cell/μl(50.7%~86.0%);CD3+CD4+细胞380~1208cell/μl(23.3%~50.2%),CD3+CD8+细胞229~982cell/μl(12.5%~36.9%),CD3+CD4+/CD3+CD8+比值0.80~2.74。结论性别等因素可影响人外周血T淋巴细胞亚群,有必要建立适当的、适用于北京地区健康成人的CD4+和CD8+T淋巴细胞亚群参考范围。
出处 《临床检验杂志》 CAS CSCD 北大核心 2009年第5期390-392,共3页 Chinese Journal of Clinical Laboratory Science
  • 相关文献

参考文献9

  • 1Guidelines for the enumeration of CD4^+ T lymphocytes in immunosuppressed individuals. CD4^+ T lymphocyte Working Party. Members of the General Haematology Task Force of BCSH[ J]. Clin Lab Haematol, 1997,19 (4) : 231-241.
  • 21997 revised guidelines for performing CD4^+ T-cell determinations in persons infected with human immunodeficiency virus (HIV). Centers for Disease Control and Prevention [ S ]. MMWR Recomm Rep, 1997,46 (RR-2) : 1-29.
  • 3Wiener D, Shah S, Malone J, et al. Multiparametric analysis of peripheral blood in the normal pediatric population by flow cytometry [J]. J Clin Lab Anal,1990,4(3) :175-179.
  • 4Erkeller-Yuksel F M, Deneys V, Yuksel B,et al. Age-related changes in human blood lymphocyte subpopulations [ J ]. J Pediatr, 1992,120 (2 Pt 1 ) :216-222.
  • 5Lee B W,Yap H K,Chew F T,et al. Age-and sex-related changes in lymphocyte subpopulations of healthy Asian subjects: from birth to adulthood[ J]. Cytometry, 1996,26( 1 ) :8-15.
  • 6Wack A, Cossarizza A, Hehai S, et al. Age-related modifications of the human alphabeta T cell repertoire due to different clonal expansions in the CD4^+ and CD8^+ subsets [ J ]. Int Immunol, 1998,10 (9) :1281-1288.
  • 7Ginaldi L,De Martinis M,D'Ostilio A,et al. Changes in the expression of surface receptors on lymphocyte subsets in the elderly: quantitative flow cytometric analysis[J]. Am J Hematol,2001,67(2) :63-72.
  • 8Rudy B J, Wilson C M, Durako S, et al. Peripheral blood lymphocyte subsets in adolescents: a longitudinal analysis from the REACH project[ J]. Clin Diagn Lab Immunol,2002,9(5 ) :959-965.
  • 9Gratama J W,Kraan J,Keeney M,et al. Reduction of variation in T- cell subset enumeration among 55 laboratories using single-platform, three or four-color flow cytometry based on CD45 and SSC-based gating of lymphocytes [ J ]. Cytometry, 2002,50 ( 2 ) : 92 -101.

同被引文献136

引证文献14

二级引证文献36

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部