运动诱导肌源性IL-6基因表达和蛋白释放与GLUT4基因表达的关系研究

Relation between exercise-induced muscle-derived IL-6 gene expression and protein release and GLUT4 gene expression

目的:研究不同肌糖原状态下运动诱导肌源性白细胞介素6(Interukin-6,IL-6)表达及蛋白释放与葡萄糖转运体4(Glucose Transport 4,GLUT4)基因表达的关系。方法:通过特定的运动方案和饮食方案造成大鼠在运动前体内肌糖原含量不同,再观察定量负荷跑台运动后肌源性IL-6表达及释放与葡萄糖转运体GLUT4基因表达的变化规律。结果:血清IL-6浓度在运动30min后显著增加,运动120min后达到峰值,运动后逐渐降低;骨骼肌IL-6mRNA在运动120min后显著增加,运动后3-6h继续增加至峰值;骨骼肌GLUT4mRNA在运动时并未增加,而在运动后3h开始增加,运动后6h达到峰值。上述三个指标在低肌糖原组的增加与正常糖原组相比更加显著。结论:低肌糖原含量可以促进运动中IL-6的释放及运动后恢复期IL-6和GLUT4的基因表达;运动导致恢复期GLUT4基因表达的增加很有可能与肌源性IL-6的表达及IL-6的自分泌作用有关。

This paper was to research into the relation between exercise-induced muscle derived IL-6 gene expression and protein release and GLUT4 gene expression in the different states of skeletal muscle glycogen caused by special glycogen and meal projects. Changes of muscle-derived IL-6 gene expression and protein release as well as GLUT4 gene expression after treadmill exercise were monitored. It was seen that serum IL 6 concentration increased in the groups of exercise in 30 minutes , then peaked in the groups of exercise in 120 minutes and decreased gradually after exercise. IL- 6mRNA of skeletal muscles increased in the groups of exercise in 120 minutes . , then peaked in the groups of post-exercise in three to six hours . GLUT4mRNA of skeletal muscles did not increase in the groups of exercise. However, it increased in the groups of post-exercise in three hours, then peaked in the groups of post-exercise in six hours . Compared with the normal glycogen groups, the three above indicators increased more significantly in the low muscles glycogen groups. It could be concluded that low muscle glycogen content could induce the release of IL-6 and GLUT4 in the recovery period after exercise. It was likely that exercise-induced increase of GLUT4 gene expression in the recovery period after exercise was related with auto-secretion of IL-6.