牛、绵羊和山羊染色体同源性分析及山羊部分毛色候选基因染色体电子定位

Homology of Chromosomes among Cattle,Sheep and Goat and Electro-localization of Goat′s Partial Coat Color Candidate Genes

旨在为牛科物种进化和山羊毛色基因染色体定位研究提供更多的理论依据。利用GOATMAP DATA-BASE和NCBI生物学数据库查询牛、山羊和绵羊常染色体上的标记,然后用Phylogenetic Computer Tools与进化树软件Phylip进行牛、绵羊和山羊染色体同源性分析;利用比较基因组学和生物信息学方法对山羊部分皮毛颜色候选基因进行定位。结果表明,山羊、牛和绵羊染色体具有较高的同源性,相应同源染色体间相似性系数在0.000 0-1.000 0;结果,山羊皮毛颜色候选基因Myo5a,Brca1、Sox10、Zic2、kit、Snai2、Wnt3a和Tyrp1分别电子定位于山羊10、17、5、12、6、14、7和8号染色体上。

The purpose of this study is to provide more theoretical basis for the research of evolution of bovine and chromosome electro-localization of some goat′s coat color genes.The homology of chromosomes among cattle,sheep and goat was analyzed using Phylogenetic Computer Tools and Phylip software based on markers located at their autosomes.Partial coat color candidate genes of goat were mapped using the method of comparative genomics and bioinformatics.The results indicated that similarity of homologous chromosomes among these species was 0.000 0-1.000 0.The coat color candidate genes of goat,Myo5a,Brca1,Sox10,Zic2,kit,Snai2,Wnt3a and Tyrp1 were oriented at chromosome 10,17,5,12,6,14,7,and 8,respectively.