玻璃化法保存异体肌腱移植的组织学研究

Morphologic study on cryopreservation of tendoh allograft by vitrification

[目的]对玻璃化法与程序冷冻法保存异体肌腱移植后的组织学演变及特性进行观察、分析,以了解其临床应用的可行性及影响因素。[方法]家兔36只,平均分为A组为新鲜自体肌腱移植组,B组为玻璃化法保存异体肌腱移植组,C组为程序冷冻法保存异体肌腱移植组,对术前、术后3、8、12周异体肌腱的形态学、组织学演变进行观察。[结果]玻璃化法保存肌腱完整率高于程序冷冻法,差异有显著性。A、B组移植后较C组粘连轻,光泽好。新生血管、腱细胞成熟早,愈合进程快。[结论]玻璃化法较好地保存了肌腱的细胞外结构,玻璃化法保存异体肌腱移植具有接近正常肌腱的组织学特性,其效果优于程序冷冻法。

[ Objective] To analyze the histological changes and mechanical characteristics after vitrification and slow-freeing method for tendon allograft. [ Method] Thirty-six rabbits were divided into 3 groups： Group A （fresh tendon autograft ）, Group B （vitrified tendon allograft）, Group C （slow-freezing tendon allograft）. The morphologic changes of tenddonial grafts were observed microscopically and histologically at 0,3,8, and 12 weeks. [Result] Before transplantation, the integrated rate of Group B was 92. 4% ,which was significantly better than that of Group C （54.2% ,P 〈 0.01 ）. After-transplantation, Group C was more heavy adhesion around tendon than that of Group A or B. In Group A and B, new collagen fibers and significantly increased and reconstructed than that of Group C. [ Conclusion] The tendon extracellular matrix could be preserved perfectly,and the tendon allograft can be preserved by vitrification. It is more applicable clinically than slow-freezing tendon allograft.