RP-HPLC法同时测定蟾麝救心滴丸中蟾酥华蟾酥毒基和酯蟾毒配基的含量

Simultaneous determination of cinobufagin and resibufogenin in Chanshe Jiuxin pills by RP-HPLC

目的建立采用RP-HPLC法同时测定蟾麝救心滴丸中蟾酥华蟾酥毒基和酯蟾毒配基含量的方法。方法采用反相HPLC法测定华蟾酥毒基、酯蟾毒配基的含量,C18色谱柱;流动相:乙腈-水,梯度洗脱(0→25min,56∶44;26→44min,0∶100;45→50min,56∶44,V/V),流速:1.0ml/min,检测波长为296nm,柱温为35℃。结果华蟾酥毒基在0.2118-2.118μg范围内呈现良好的线性关系(r=0.9997),平均回收率为99.49%,RSD值为1.2%(n=6);酯蟾毒配基在0.2012-2.012μg范围内,呈现良好的线性关系(r=0.9996),平均回收率为99.97%,RSD值为1.7%(n=6)。结论RP-HPLC法简便可行、准确、重现性好,可作为蟾麝救心滴丸中蟾酥华蟾酥毒基和酯蟾毒配基的含量测定方法。

Objective To establish a method for determination of cinobufagin and recibufogenin in Chanshe Jiuxin pills by RP-HPLC. Methods Kromasil C18 column was used,with acetonitrile-water（0→25 min,56：44;26→44 min,0：100;45→50 min,56： 44, V/V） gradient elution as a mobile phase at a flow rate of 1.0 ml/min. The wavelength of detection was 250 nm. The column temperature was 35 ℃. Results The determination of cinobufagin showed a good linear relationship in the range of 0.211 8 -2.118 μg （r = 0.999 7 ） ,with the average recovery of cinobufagin 99.49% and RSD 1.2% （ n = 6）. The determination of recibufogenin showed a good linear relationship in the range of 0. 201 2 - 2. 012 μg（ r = 0.999 6）. The average recovery of recibufogenin was 99.97% , and RSD was 1.7% （n = 6）. Conclusion The method is simple,accurate and precise,which can be used for the assay of Chanshe Jiuxin pills.