莨山唇柱苣苔组织培养与植株再生

Tissue Culture and Plant Regeneration in Chirita langshanica

为了能快速繁殖莨山唇柱苣苔,以其嫩叶为试验材料,进行了在不同培养条件下的组织培养研究。研究结果表明:MS+BA 1.0 mg.L-1+NAA 0.5 mg.L-1为愈伤组织诱导的最佳培养基;MS+BA 0.5mg.L-1+NAA 0.3 mg.L-1为丛生芽分化的最佳培养基;MS+NAA 0.3 mg.L-1+石英砂为最佳生根培养基。在80%遮阳网下进行炼苗,移栽25 d后成活率可达80%。

In order to realize rapid propagation in Chirita langshanica,taking tender leaves as experimental materials,tissue culture techniques in Chirita langshanica were studied under different culture conditions.The results indicated that MS supplemented with BA 1.0 mg·L-1 and NAA 0.5 mg·L-1 was the optimal medium for inducing callus,MS supplemented with BA 0.5 mg·L-1 and NAA 0.3 mg·L-1 was the best medium for cluster buds differentiation,and MS supplemented with NAA 0.3 mg·L-1 and quartz sand was the optimal medium for inducing root. Hardening seedlings should be carried out under 80% shading-net, and the survival rate of seedlings replanted was up to 800/00 after 25 days.