摘要
目的对2009年1月15日山东省济南市发生的1例人禽流感病例进行实验室诊断过程分析,探讨实验检测过程中遇到的问题。方法采集病人咽拭子和呼吸道吸取物标本,利用RT-PCR和real-time RT-PCR检测病毒核酸。结果第2次采集的咽拭子标本采用H5HA引物的RT-PCR扩增呈阳性;呼吸道吸取物分别用H5NA等3对引物进行RT-PCR均阳性,real-time RT-PCR阳性。结论该患者为人感染高致病性禽流感病毒(H5N1)实验室确诊病例。呼吸道吸取物是禽流感病毒核酸检测的合适标本。
Objective To analyze the laboratory diagnosis of a human case of Avian Influenza Virus (H5N1) in Jinan, Shandong Province in Jan 15, 2009 and discuss problems with the diagnostic process. Methods The patient's respirato- ry tract samples and throat swabs were tested with regard to nucleic acids using RT-PCR and real-time RT-PCR. Results The results of nucleic acid detection of the second throat swab were positive according to RT-PCR with the specific HSNA primer; the results of nucleic acid detection of all respiratory tract samples were positive according to RT-PCR and real- time RT-PCR with three specific primers (M, HSHA and HSNA primers). Conclusion The case was confirmed to be a human case of Avian Influenza A (HSN1) virus infection. Respiratory tract samples were appropriate samples for the nucleic acid detection of the avian influenza virus.
出处
《中国病原生物学杂志》
CSCD
2009年第9期649-651,666,共4页
Journal of Pathogen Biology
关键词
禽流感
H5N1亚型
不明原因肺炎
实验室检测
Avian influenza
H5N1 subtype
atypical pneumonia case
laboratory diagnosis