急性心肌梗塞循环内皮祖细胞与冠状动脉病变严重程度的分析

Correlation between level of circulating endothelial progenitor cells and severity of coronary artery disease in patients with acute myocardial infarction

目的:研究急性心肌梗塞(AMI)患者外周血中内皮祖细胞(EPCs)的水平与冠状动脉病变程度之间的关系。方法:选取55例AMI患者,以定量冠状动脉造影评估冠状动脉的血管狭窄程度,同时选取30名冠状动脉造影阴性的患者为对照组。所有患者均在入院后即刻[AMI发病平均时间(2.5±1.5)h],第24h、48h、72h、7d、14d及一个月时采血,以CD133作为EPCs标记物,用流式细胞仪检测患者外周血中CD133标记细胞数量。结果:AMI组患者及对照组均有EPCs(CD133)的表达,AMI患者EPCs数目明显低于非冠心病患者(P<0.05)。多支病变者较单支病变者有降低趋势,但无显著性差异(P>0.05)。EPCs数目和Gensini评分呈明显负相关(n=55,r=-0.619,P<0.05)。结论:急性心肌梗塞患者EPCs数目和冠状动脉病变程度有关。

Objective： To research the correlation between level of circulating endothelial progenitor cells and severity of coronary artery disease in patients with acute myocardial infarction. Methods： A total of 55 acute myocardial infarction （AMI） patients who underwent coronary angiography were enrolled. The coronary vascular stenosis was evaluated with quantitative coronary angiography. At the same time the 30 patients with normal coronary angiography were regard as control group. All patients were bled immediately [AMI average time of （2.5±1.5） h] , on 24 h, 48 h, 72 h, 14 d and one month. EPC was marked by CD133, and flow cytometry was used to detect cell marked with CD133. Results： EPCs （CD133） were both expressed in peripheral blood of AMI patients and control group. The number of EPCs in AMI patients was significantly lower than that of non-coronary heart disease （P〈 0.05）. The number of EPCs in patients with multi-vessel disease was lower than that with single-vessel disease, but it did not reach statistical difference （P〉 0. 05）. The number of EPCs was clearly negative correlation with Gensini score （n = 55, r=0. 619, P〈0.05）. Conclusion： Number of EPCs are correlated with the extent of coronary artery disease in patients with acute myocardial infarction.