HPLC法测定牙膏中柚皮苷的含量

Determination of naringin in toothpaste by high performance liquid chromatography

建立了高效液相色谱测定牙膏中柚皮苷含量的方法。采用的色谱条件:Hypersil BDS C18色谱柱(250 mm×4.6 mm,5μm);柱温为40℃;以水(A相)和乙腈(B相),梯度洗脱程序为:0～15 min,10%～100%B;流速为1.0 mL/min;检测波长为283 nm;进样量为20μL。结果表明,柚皮苷的质量浓度在14.55～116.40μg/mL范围内与峰面积呈良好的线性关系(r=0.9999),平均加标回收率为97.56%。该方法稳定、准确,重现性好,可作为牙膏中柚皮苷的含量测定和质量控制方法。

A high performance liquid chromatographic method was developed for determining the content of Naringin in toothpaste. The chromatographic analysis was carried out on a Hypersil BDS C18 column（250 mm×4, 6 mm, 5 μn）. The mobile phase was water（A）and acetonitrile（B）with gradient elution（0- 15 min, 10%- 100% B）. The flow rate was maintained at 1.0 mL/min; The detection wavelength was set at 283 nm and the column temperature was controlled at 40 ℃ ,The sample injection volume was 20 μL. The results showed that the mass concentration of Naringin with good linearity vs. the peak area within ranges of 14.55-116.40 μg/mL,r=0. 9999. The average recovery was 97.56 %. The method was proved to be stable, reproducible and precise,It could be used for determination and quality control of naringin in toothpaste.