摘要
于发情周期的第10天,给12头参试母牛肌注PMSG(孕马血清促性腺激素,总剂量为1350~2000IU)。每头参试牛于肌注PMSG前的当天,肌注后24h、48h,肌注氯前列烯醇后24h、48h,发情后48h,排卵或妊娠5、10、20、60d,从颈静脉各采血1次,共采10次,每次10ml,离心后分离血清。用放免法测定上述各生理时期血清中P4(孕酮)、LH(促黄体素)、E2(雌二醇)水平。结果表明,适量的PMSG(1350~2000IU)能诱导母牛产双胎,其双胎率为42.9%(3/7)。测定早期妊娠母牛(5、10、20、60d)血清中P4水平,证明双胎牛均高于单胎牛。
Twelve cows were injected intramuscularly(i m )with PMSG at 1 350 IU to 2 000 IU on the 10th day of the estrus cycle.Blood was collected from cows’ jugliar vein on the 10th day of the cycle,at 24 h and 48 h after injection with PMSG,at 24 h and 48 h after i m injection with cloprostenol,at 48 h after estrus,and on 5,10,20 and 60 d after ovulation or pregnancy.Serum was prepared and the levels of P 4,LH and E 2 in serum of the cows were measured by RIAs.The results showed that PMSG could induce twin pregnancy of the cows,twinning rate being 42 9%(3/7).Average levels of P 4 in twin pregnancy cows on 5,10,20 and 60 d after pregnancy were higher than those of single pregnancy cows.
出处
《畜牧与兽医》
北大核心
1998年第6期250-252,共3页
Animal Husbandry & Veterinary Medicine