TRAIL联合顺铂和IFN-γ对人骨肉瘤细胞凋亡的影响

Effect of TRAIL Combined with Cisplatin and IFN-γ on Apoptosis of Osteosarcoma Cells

目的:探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)联合顺铂和IFN-γ应用对人骨肉瘤细胞生长抑制和诱导凋亡的作用。方法:用MTT测定TRAIL、顺铂、IFN-γ单独或联合应用对人骨肉瘤细胞MG-63的体外增殖抑制作用,并检测各组细胞凋亡率。结果:TRAIL、顺铂、IFN-γ三者联合作用于MG-63细胞后,能显著增强对MG-63的杀伤、抑制增殖及诱导凋亡作用,明显高于单独应用TRAIL组或顺铂组(P<0.05)。结论:TRAIL、顺铂、IFN-γ三者联用能显著提高骨肉瘤细胞MG-63生长抑制和诱导凋亡作用。

Objective：To investigate the influence of tumor necrosis factor related apoptosis inducing ligand （TRAIL） combined with cisplatin （DDP） and IFN-γ on the proliferation and apoptosis of osteosarcoma cell MG-63. Methods： MTT assay was used to evaluate the cytotoxie effect on MG-63. Flow cytornetric analyses were performed to detect apoptosis rates. Results：The combined therapy induced significantly higher apoptosis and cytotoxicity than TRAIL, DDP or IFN-γ alone （P〈0.05）. Conclusion：The combination of TRAIL, cisplatin and IFN-γ could significantly increase cytotoxic and apoptosis effect on MG-63.