双氢青蒿素干预结直肠癌模型动物肿瘤生长的实验研究

Experimental Study on the Intervention of Dihydroartemisinin on Colorectal Cancer Growth in Animal Models

【目的】观察双氢青蒿素(DHA)对结直肠癌模型动物肿瘤生长的影响。【方法】选用SPF级BALB/c裸鼠,背部皮下接种结直肠癌LoVo细胞悬液复制结直肠癌模型;造模成功裸鼠随机分成5组:模型对照组,5-FU组(腹腔注射5-FU20 mg.kg-1.d-1,共5 d),DHA高、中、低剂量组(分别按120、60、30 mg.kg-1.d-1剂量灌胃给药)。14 d后,检测活体肿瘤及解剖剥离后裸瘤体积、平均瘤体质量、肿瘤生长速率和抑瘤率。【结果】治疗后DHA各给药组及5-FU组活体肿瘤的体积及肿瘤生长速率均减少,与模型组比较差异有显著性意义(P<0.01);5-FU组及DHA高、中剂量组的裸瘤体积显著小于模型组(P<0.05或P<0.01);各给药组瘤体质量显著低于模型组(均P<0.05);DHA高、中剂量组的抑瘤率显著高于5-FU组(P<0.05),但DHA低剂量组抑癌率显著低于5-FU组(P<0.05)。【结论】DHA对结直肠癌模型动物肿瘤生长具有一定的抑制作用。

Objective To observe the influence of dihydroartemisinin（DHA） on colorectal cancer growth in animal models.Methods Colorectal cancer animal models were established by subcutaneous injection of colorectal cancer LoVo cells suspension into the back of specific-pathogen free BALB/c nude mice.Then the BALB/c tumor-bearing nude mice were randomized into 5 groups： model control group,5-FU group（intraperitoneal injection of 5-FU 20 mg·kg-1·d-1 for 5 days）,and high-,middle-and low-dose DHA groups（gastric gavage of DHA in the dose of 120,60 and 30 mg·kg-1·d-1 respectively for 14 days）.The in-vivo and in-vitro size of tumor mass,mean tumor mass weight,tumor growth rate and tumor-inhibition rate were examined after treatment.Results After treatment,the in-vivo size of tumor mass and the tumor growth rate were decreased in 5-FU group and DHA groups（P〈0.01 compared with those in the model group）;in-vitro tumor mass size was reduced in 5-FU group and in high-and middle-dose DHA groups（P〈0.05 or P〈0.01）;tumor mass weight in the medication groups was less than that in the model group（P〈0.05）;the tumor-inhibition rate was higher in high-and middle-dose DHA groups but lower in low-dose DHA group than that in 5-FU group（P〈0.05）.Conclusion DHA exerts certain inhibitory effect on tumor growth in colorectal cancer animal models.