草珊瑚离体不定丛芽诱导因子筛选的研究

Selection of Induction Factors for Adventitious Buds of Sarcandra glabra in Vitro

【目的】探讨影响草珊瑚不定芽诱导的培养因素,为建立草珊瑚初代离体培养体系提供依据。【方法】以草珊瑚下胚轴诱导获得的无菌再生苗茎段为外植体,考察6-苄氨基嘌呤(6-BA)和吲哚丁酸(IBA)不同浓度配比、光照时间和强度以及温度对草珊瑚离体不定芽高频诱导的影响。【结果】6-BA和IBA组合能有效提高草珊瑚不定芽的诱导率,而高浓度的植物激素会抑制草珊瑚不定芽的生长;草珊瑚在散射光或弱直射光照射下生长势头好,暗培养或过强直射光会出现植株发黄、皱缩、甚至死亡的现象;在温度为25℃时,草珊瑚不定芽诱导率达到100%,低于25℃或高于30℃均不利于其生长。【结论】以MS培养基为基本培养基,附加2.5 mg/L 6-BA+0.1 mg/L IBA,温度为25℃,采用柔弱自然散射光或低于2 000 lx直射光照射,时间少于8 h能有效促进草珊瑚离体不定芽的诱导,诱导率达82.98%。

Objective To establish the culture technology system for the in-vitro induction of adventitious buds of Sarcandra glabra（Thumb.） Nakai by investigating the induction factors of in-vitro adventitious buds.Methods Stem segments of Sarcandra glabra were cultured as explants.The effects of culture medium with different concentrations of 6-benzylaminopurine（6-BA） and indole butyric acid（IBA） added,lighting time,light intensity,and temperature on the induction of adventitious buds from the explants were observed.Results The medium containing both 6-BA and IBA was more effective to induce shoot formation of Sarcandra glabra than the medium only containing 6-BA or IBA;high concentration of plant hormones would block the growth of the plantlets.The scattering light or faint direct light was suitable for the shoot formation,while dark or strong light would induce the plantlets withered or even dead.Temperature in the range of 25 ℃～30 ℃ was beneficial for the growth of adventitious buds,and the bud induction rate arrived 100% at the temperature of 25 ℃.Conclusion At the temperature of 25 ℃,the faint sunlight or direct light less than 2000 lux,and the medium with 2.5 mg·L-1 6-BA and 0.1 mg·L-1 IBA added can stimulate significantly the adventitious bud formation of Sarcandra glabra,the induction rate up to 82.98%.