湿热痹片对佐剂性关节炎大鼠T细胞及细胞因子网络的调节作用

Regulation Effect of SHI-re-bi Tablet on T Lymphocyte and Cytokine Network in the Adjuvant-induced Arthritis Rats

目的:观察湿热痹片对灭活结核分枝杆菌(Mtb)诱导的佐剂性关节炎大鼠T细胞及细胞因子水平的影响。方法:采用热杀死结核分枝杆菌(H37Ra Mtb)免疫SD大鼠,以诱导佐剂性关节炎,用ELASA法检测大鼠外周血T淋巴细胞亚群、肿瘤坏死因子α(TNF-α)水平;同时测定大鼠踝关节滑膜组织中IL-1β,IL-6,IL-10及血管内皮细胞生长因子(VEGF)水平的变化。结果:与模型对照组比较,湿热痹组CD8+T细胞水平升高(P<0.05),CD4+/CD8+比值降低(P<0.05);促炎细胞因子TNF-α,IL-1β及IL-6水平下调(P<0.01),VEGF含量降低(P<0.01),同时抗炎因子IL-10水平上升(P<0.01)。结论:湿热痹片通过调节T细胞及细胞因子网络平衡而发挥对类风湿关节炎的治疗作用。

Objective： To observe the influence of Shi-re-bi Tablet on T lymphocyte and cytokine network in the adjuvant-induced arthritis SD rats.Methods： Induce the model of adjuvant arthritis in SD rats through the immunization of heat-killed Mycobacterium Tuberculosis H37RA（Mtb）.Measure the level of TNF-α and T lymphocyte subsets in the experimental animal＇s peripheral blood by ELISA.At the same time,measure the level of IL-1β,IL-6,IL-10 and VEGF in the synovial tissues of ankle joint in the rheumatoid arthritis rats.Results： Compared with the model group,Shi-re-bi Tablet could raise the level of CD8＋ T lymphocyte（P〈0.05） and reduce the ratio of CD4＋/CD8＋（P〈0.05）,proinflammatory TNF-α,IL-1β and IL-6（P〈0.01）,at the same time,the content of VEGF is reduced（P〈0.01） and the level of anti-inflammatory IL-10 is raised（P〈0.01）.Conclusions： Shi-re-bi Tablet can regulate the balance of T lymphocyte and cytokine network,and thus may be its mechanism for treatment of rheumatoid arthritis.