中药复方清热颗粒剂抗急性耐药菌感染的药效作用研究

Pharmacodynamic Effect of Compound Qingre Granules on Acute Drug-resistance Bacterial Infection

目的:观察中药复方清热颗粒剂对急性耐药菌感染药效作用。方法:新西兰大耳白兔腹腔注射肺炎克雷伯杆菌(ATCC700603)建立急性耐药菌感染兔动物模型,随机分为5组:正常对照组(A组)、模型组(B组)、中西药组(复方清热颗粒剂+头孢地尼,C组)、西药组(头孢地尼,D组)、中药组(复方清热颗粒剂,E组)。造模成功后连续5d各治疗组给药2次/d,A,B组给予等量的蒸馏水。观察治疗前后生命体征、血象、免疫功能、炎症损伤及病理组织形态学变化。结果:中药复方清热颗粒剂降低急性耐药菌感染动物体温(T)、呼吸频率(R),降低血浆内毒素I、L-10和TNF-α水平的作用与西药头孢地尼相同(P>0.05);中西药组T,R,腹腔菌落计数疗后恢复正常,与正常对照组比较无统计学差异(P>0.05),病死率为0%,疗效优于单纯中药、西药组(P<0.05),中西医结合疗效显著提高。结论:复方清热颗粒剂能够改善急性耐药菌感染动物的一般情况,降低血浆内毒素,调节抗炎、促炎细胞因子的含量,提高中性粒细胞吞噬指数,减轻模型动物脏器组织的病理损伤,降低病死率。

Objective To observe the pharmacodynamic effect of Compound Qingre Granules（QRKL） on acute drug-resistance bacterial infection in rabbit model.Methods Applying abdominal injection of ATCC700603,we created the acute drug-resistance bacterial infection of rabbit model and divided them into five groups： normal（A）,model（B）,QRKL＋ Cefdinir Capsules（C）,Cefdinir Capsules（D） and QRKL（E）.We observed and recorded rabbits＇ vital signs,hemogram,immune function and inflammatory mediators,etc.Patho-samples were obtained after being sacrificed.Results QRKL has the significantly pharmacodynamic effect of treating acute drug-resistance bacterial infection.There are more significant effect through integrating traditional Chinese medicine and antibiotic together.Conclusion QRKL can significantly improve the infected rabbits＇ general conditions,decrease plasma endotoxin level,and regulate cytokines of the acute drug-resistance bacteria infection,while raises nonspecific immunity so as to control the inflammatory reaction and tissue damage and lower the mortality.