柴黄复方颗粒抗炎镇痛作用及机制研究

Anti-inflammatory and Analgesic Effects of Chaihuang Compound Granule and Its Mechanism

目的:观察柴黄复方颗粒抗炎镇痛作用及其作用机制。方法:采用角叉菜胶致大鼠足肿胀法、醋酸致小鼠毛细血管通透性增加法、羧甲基纤维素钠(CMC-Na)致小鼠腹腔白细胞数增多法评价药物的抗炎作用及其机制;采用热板致痛法和醋酸致痛法评价药物的镇痛作用;用Elisa法测定炎性组织中白介素1(IL-1)和肿瘤坏死因子α(TNF-α)含量,放免法检测前列腺素E2(PGE2)的含量,紫外分光光度法测定丙二醛(MDA)含量。结果:柴黄复方颗粒能抑制大鼠足肿胀程度和CMC-Na所致小鼠腹腔白细胞游走,降低肿胀足中IL-1,TNF-α,PGE2和MDA的含量及小鼠毛细血管通透性,对抗热板和醋酸刺激引起的小鼠疼痛反应。结论:柴黄复方颗粒具有一定的抗炎和镇痛作用,其作用机制与降低炎性组织中的IL-1,TNF-α,PGE2和MDA的含量有关。

Objective：To observe anti-inflammatory and analgesic effects of Chaihuang Compound Granule（CHCG） and its mechanism.Methods：Carrageenan-induced rat paw edema model in rat,acetic acid-induced capillary permeability increase in mouse and sodium carboxy-methylcellulose（CMC-Na）-induced leukocytes increase in mouse were used to study the anti-inflammatory effects.The analgesic effects of CHCG were tested by counting body twisting induced by acetic acid and measuring the latent period of licking hind foot with the hot plate method in mice. The content of IL-1,TNF-α,PGE2 and MDA were measured by ELISA,radio-immunity method and ultraviolet spectrophotometry respectively.Results：CHCG significantly suppressed the carrageenan-induced rat paw edema and leukocyte migration caused by CMC-Na;The content of IL-1,TNF-α,PGE2 and MDA in the inflammatory tissue extracts was significantly decreased,the capillary permeability of mice was also decreased.CHCG also improved the pain threshold of hot-plate test in mice and those injected with acetic acid.Conclusion：The anti-inflammatory and analgesic effects of CHCG are obvious and it might be associated with the decrease of IL-1,TNF-α,PGE2 and MDA.