穿心莲对糖尿病大鼠血管病变保护机制的研究

Vascular Protecting Effects of Androgrpahis paniculata Nees for Diabetes Mellitus in Rat

目的:探讨穿心莲对糖尿病血管病变的保护机制。方法:制备糖尿病大鼠模型,并给予穿心莲干预,8周后,取血测定一氧化氮(NO)及内皮素-1(ET-1)。取胸主动环观察不同累积浓度的乙酰胆碱(Ach)对去甲肾上腺素(NE)引起的血管收缩的抑制率。另留取一段胸主动脉制备病理切片,SP法免疫组化染色,观察细胞间黏附分子-1(ICAM-1)及血管细胞间黏附分子-1(VCAM-1)的阳性表达。结果:穿心莲组与模型组相比能明显抑制升高的血浆ET-1(P<0.05);升高NO的水平(P<0.05);但均达不到正常组的水平(P<0.05)。模型组血管环对NE的收缩反应明显强于正常对照组及穿心莲组(P<0.05),且穿心莲组对NE的收缩反应亦强于正常对照组(P<0.05)。在相同Ach浓度下,模型组及穿心莲组胸主动脉环对Ach的舒张作用明显弱于正常组(P<0.05),而穿心莲组强于模型组(P<0.05)。穿心莲组能明显抑制糖尿病大鼠胸主动脉ICAM-1及VCAM-1表达(P<0.05)。结论:穿心莲具有降低大鼠ET-1及升高NO的作用,能够保护糖尿病大鼠内皮依赖的血管舒张功能,且能够抑制ICAM-1及VCAM-1的表达,具有抗动脉硬化作用。

Objective：To explore the protective mechanism of Chinese Herb-Androgrpahis paniculata Nees on the vascular disease of diabetes mellitus（DM） in rat.Methods： The DM rat model was made.Androgrpahis paniculata Nees was given to treatment group.8 weeks later,blood sample was taken to test nitric oxide（NO）and endothelin-1（ET-1）.Thoracic aortic ring was employed to observe the inhibition by different concentration of Ach to constraction caused by NE.Another part of aorta was used to make slices,SP immunohistochemicae staining was employed to observe the expression of ICAM-1 and VCAM-1.Result： Androgrpahis paniculata Nees group obviously decreased plasma ET-1 and increased the level of NO comparied with Diabetes group （P〈0.05）,but the parameters could not reach the level of the normal control group（P〈0.05）.The expression of ICAM-1 and VCAM-1 could be obviously inhibited in Androgrpahis paniculata Nees group comparied with diabetes group.But the parameters were obviously higher than normal control group（P〈0.05）.Conclusion： Chinses medicine Androgrpahis paniculata Nees could decrease the level of ET-1 and increase the level of NO in diabetes rats,they have the function of protecting the endothelium of vessel,and could inhibte the expression of ICAM-1 and VCAM-1,acting as the role of anti-atherosclerosis.