猪EPOR和MYPN基因SNPs检测及其与肉质、胴体性状关联分析

SNPs Detection of EPOR&MYPN Gene and Association with Meat Quality Traits in Porcine

对EPOR、MYPN2个候选基因进行SNPs检测,并与肉质性状的关联分析,以期为分子育种提供依据。采用PCR-SSCP及PCR-RFLP方法对目的基因片段进行SNPs检测,结果显示,在研究群体内,EPOR基因不存在SNP,而MYPN基因存在2个SNP。用SAS软件对MYPN基因的2个SNP位点与部分肉质、胴体性状进行关联分析,结果表明,在本试验群体中,SNP1对背膘厚、导电性、眼肌面积和肌内脂肪的影响都达到了显著水平(P<0.05);SNP2对其中的背膘厚和肌内脂肪含量影响达到了显著水平(P<0.05);另外性别、品种对部分性状也有较大影响(P<0.05)。结合本研究结果和其它相关研究结果,可以推断MYPN基因可能是影响肉质、胴体性状的主效基因或与控制这些性状的主效基因连锁,可以作为肉质、胴体性状的候选基因。

The two genes EPOR and MYPN were analyzed by SNP genotyping and associated with meat quality traits to find the candidate genes for meat quality traits. The methods for SNPs genotyping are PCR SSCP and PCR-RFLP. No SNP were found for EPOR genes. Two SNPs were found in MYPN genes and the two genes were associated with meat quality traits by SAS. The results showed that SNP1 had significant effects on back fat thickness, conductivity, eye muscle area, intramuscular fat （P〈0.05）; SNP2 had significant effects on back fat thickness and intramuscular fat （P〈0.05）;sex and breed had significant effects on some traits （P〈0. 05）. Combined results of this study and other related research findings, it may be inferred that the MYPN gene may affect the meat quality traits and carcass traits, or the major gene and control of these traits in the main chain gene can be used as meat quality carcass traits of the candidate genes.