小麦粘类CMS育性恢复基因的SSR分子标记与定位

MAPPING OF FERTILITY RESTORING GENE FOR MALE STERILITY WITH Ae.kotschyi,Ae.variabilis, Ae.ventricosa CYTOPLASMS BY USING SSR MARKERS IN WHEAT

利用SSR技术,对小麦粘类CMS(细胞质雄性不育)的1对近等基因系(NILs)和回交群体(BC’1)进行分析,筛选与粘类CMS育性恢复基因相连锁的分子标记并进行恢复基因定位,以进一步探讨小麦粘类CMS育性恢复的遗传机理。结果表明:在18对位于1BS上的SSR引物中,有6对引物在近等基因系间扩增出了稳定的多态性差异;经分离群体验证表明,恢复基因与4对SSR引物的扩增位点Xwmc406、Xbarc8、Xwmc611和Xgwm273有连锁关系,遗传距离分别为2.7、2.8、21.4和26.2cM,这些标记可稳定应用于小麦粘类CMS育性恢复基因的辅助选择;研究还表明,小麦粘类雄性不育系的育性恢复主要受1BS上的1对主效恢复基因及一些微效基因共同控制;本研究标记出的恢复基因应为Rfv1;上述4个标记与该主效恢复基因Rfv1之间的位置顺序依次为Xwmc406、Rfv1、Xbarc8、Xwmc611、Xgwm273。

Microsatellite markers were employed to map the major restoring gene for male sterility with Ae.kotschyi, Ae.variabilis, Ae.ventricosa cytoplasms by using materials of NILs and BC＇_1 to probe into the genetic mechanism of fertility restoration. The results showed that 18 sets of SSR primers which were located on the chromosome 1BS of wheat were screened for polymorphism between the NILs, and 6 of which were found polymorphic. Linkage analysis indicated that Xwmc406,Xbarc8,Xwmc611 and Xgwm273 were linked to the restoring gene. The breeding for new fertility restorer lines of male sterility with Ae.kotschyi, Ae.variabilis, Ae.ventricosa cytoplasms would be facilitated by using these polymorphic markers. The result also showed that the fertility restoration of male sterility with Ae.kotschyi, Ae.variabilis, Ae.ventricosa cytoplasms was mainly controlled by both major fertility restoring gene and some minor enhance genes of fertility. The restoring gene which was marked in this research should be Rfv1. The distances between the restoring gene and the 4 markers were 2.7, 2.8, 21.4 and 26.2cM, and the sequence of the 4 markers and Rfv1 were Xwmc406, Rfv1, Xbarc8, Xwmc611 and Xgwm273.