摘要
用剂量分别为15、20、25、30和35Gy的60Co γ射线辐照处理晚香玉单瓣、重瓣2个品种的种球,以未辐照种球为对照,以通过60Coγ射线辐照处理筛选出优良品种。结果表明:各处理对植株的生长和发育均有影响,主要表现在出苗率降低,植株变矮,开花延迟,花期延长。15和20Gy处理组的植株辐照损伤较小,植株长势相对较好,尤以20Gy处理的植株更为突出,2个品种间单瓣的耐辐照能力较重瓣强。运用聚丙烯酰胺凝胶电泳(PAGE)对不同剂量辐照下的2个品种的植株叶片进行同工酶分析,结果显示,在20和25Gy剂量下,过氧化物酶、酯酶和过氧化氢酶的活性最强。经农艺性状及同工酶分析,初步筛选出2个晚香玉品种的适宜辐照剂量为20Gy。
The tubers of 2 tuberose varieties with single and double petal were irradiated by ^60Co γ-rays at different dose（15,20,25,30 and 35 Gy）and the untreated tubers used as control. The results showed that the irradiation had affect the plant growth and development, such as reduced emergence rate,shortened plant height, late flowering and florescence prolonged. The damage of plants treated by 15 and 20 Gy were less, especially the 20 Gy. The tuberose with single petal was more tolerant to irradiation than the double petal variety. Through the analysis of isoenzyme by poly acrylamide gel electrophoresis, we found that 20 and 25 Gy treatments could promote the expression of peroxide isoenzyme, ester isoenzyme and catalase isoenzyme. According to the results of agronomic traits and isoenzyme analysis,the dose of 20 Gy was suitable for two varieties of Polianthes mutagenic L.
出处
《核农学报》
CAS
CSCD
北大核心
2009年第5期799-804,共6页
Journal of Nuclear Agricultural Sciences
基金
安徽省高等学校学科带头人基金项目(2005008)
安徽农业大学稳定和引进人才科研项目(WD2007006)
关键词
晚香玉
^60CO
γ射线
辐照剂量
过氧化物酶
酯酶
过氧化氢酶
Polianthes tuberose L.
^60Co γ-rays
irradiation dose
peroxide isoenzyme
ester isoenzyme
catalase isoenzyme
