痰湿证大鼠模型肝脏脂质合成基因的表达

The Liver Lipogenic Gene Expression of Animal Model of Phlegm and Dampness Syndrome

目的观察高脂喂养制作的痰湿证大鼠模型肝脏脂质合成相关基因的表达。方法根据“肥白人多痰湿”的理论，采用高脂饮食喂养法建立痰湿证大鼠模型，采用肝脏HE染色观察模型大鼠肝脏的病理改变，检测血清和肝脏的三酰甘油（TG）和游离脂肪酸（FFA）水平，采用荧光实时定量PCR技术检测肝脏X受体α（LXRα）、固醇调节元件结合蛋白-1c（SREBP-1c）、脂肪酸合酶（FAS）等与脂质合成相关的基因表达。结果模型大鼠肝脏出现大量含有脂滴的空泡，血清、肝脏脂质含量与正常组相比显著增高，LXR的表达无显著变化，SREBP—1c和FAS的表达增高。结论模型大鼠肝脏的脂肪沉积，与脂质合成基因的表达增强有关。

Objective To observe The liver lipogenic gene expression of animal model of phlegm and dampness syndrome. Methods According to the theory ＂Obese people have much more phlegm and dampness＂, the animal model of phlegm and dampness was built by high fat feeding. After 10 weeks＇ high fat feeding, we examined liver HE slice, the level of lipids in the normal and model group rats .The liver lipogenie gene expression including LXRα, SREBP-1c, and FAS were detected by fluorescence real time quantative PCR. Results Compared with that of the normal group rat, there was increasing lipids both in serum and liver in the model rats. There was typical appearance of fatty accumulation in the liver cells examined by liver HE staining. The expression of SREBP-1c and FAS was much higher in phlegm and dampness group than that in the normal group, although there was no difference in the expression of LXRα. Conclusion The fatty accumulation of liver cell in phlegm and dampness group has a close relation to the enhancement of the liver lipogenie gene expression.