咳尔康口服液体外抗病毒作用的研究

Study on the Antivirus Effects of Keerkang Oral Liquid on Extracorporeal Bacteriostasis

目的探讨咳尔康口服液在细胞培养中对腺病毒3型（ADV3）、副流感病毒（HVJ），呼吸道合胞病毒（RSV）、单纯疱疹病毒1和2型（HSV-1，HSV-2）的抑制作用。方法用敏感细胞培养法，采用鸡胚肾细胞（CEK）、Vero细胞，幼地鼠肾（BHK）细胞感染相应病毒2h给药，观察细胞病变（CPE）。结果当病毒感染量≤100TCID50时，咳尔康口服液组无CPE，而病毒对照组有25％-75％的CPE。咳尔康口服液抑制ADV3、HVJ、RSV、HSV-1、HSV-2的最大无毒浓度（TD0）、半数有效浓度（IC50）、最小有效浓度（MTC）和治疗指数（TI）分别为0．5、0．25、0．12540．0625ml／ml和4、8。结论咳尔康口服液在细菌培养中可显著抑制ADV3、HVJ、RSV和HSV-1，为治疗ADV3、HVJ、RSV和HSV-1感染提供了实验依据。

Objective To analyze the inhibitive effect of Keerkang Oral Liquid on adenovirus （ADV3） , parainfiuenza virus （HVJ） , respiratory syncytial virus （RSV） , and herpes simplex virus 1 （HSV-1） , herpes simplex virus 2 （HSV-2） in cell culture. Methods Sensitive cell culture was adopted, and chick embryo kidney （ CEK ） cells and bit hamster kidney （BHK） cells were used to infeet homologous virus. Keerkang Oral Liquid was given after 2 hours, then cytopathic phenomenon （CPE） was observed. Results When the amounts of infected virus are less than or equal to 100TCID50, the group which was given Keerkang Oral Liquid showed CPE, while the virus control group showed 25%-75% CPE. Besideds Keerkang Oral Liquid showed inhibitive effect on maximum non-venom concentration （TD0） , medium effective concentration （IC50） , minimum valid concentration （MTC） and therapeutic index （TI） of ADV3, HVJ, RSV, HSV- 1, and HSV-2 by 0.5, 0.25, 0.125-0.0625 ml/ml and 4.8 respectively. Conclusion Keerkang Oral Liquid has obvious inhibitive effects on ADV3, HVJ,RSV and HSV-1, HSV-2 in cell culture, which provides experimental basis for treating ADV3, HVJ, RSV and HSV- 1, and HSV-2.