地衣芽孢杆菌ws-6产纤维素酶条件优化

Optimization of Cellulase Production Conditions for Bacillus Licheniformis ws-6

目的:通过优化该菌产酶条件,以期在饲料中的进一步应用建立基础。方法:纤维素酶活性用还原糖法测定,产酶条件采用单因素筛选与正交优化方法相结合。结果:培养基初始pH6.5、培养温度37℃、接种量2%2、50 mL三角瓶装液量30 mL、葡萄糖1.5%、羧甲基纤维素钠0.5%、酵母膏1.5%、KH2PO40.05%、NaCl 0.5%,培养48h后酶活性达到最高3.45U/mL;金属离子K+、Mn2+、Mg2+、Zn2+、Co2+、Na+对产酶有激活作用,Cu2+则抑制酶活性。结论:酶活性比出发菌提高约1.4倍,对饲料制作中提高纤维降解具有一定的实用价值。

Objective： To develop further application in the feed by optimizing the production conditions of cellulase produced by Bacillus Licheniformis ws-6.Method： Cellulase activity measured by reducing sugar content,combinded with single factor test and orthogonal optimization to optimize the production conditions of cellulase.Result： The maximum activity was recorded after 48h when cultured in 1.5%glucose,0.5% carboxymethyl cellulose sodium,1.5% yeast extract,0.05% KH2PO4 and 0.5%NaCl,with 2%inoculation amount,medium volume 30mL/flask（250mL）,the initial pH was 6.5,the temperature was 37℃.Meanwhile the studies showed that Na＋,K＋,Mn2＋,Mg2＋,Zn2＋,Co2＋ could active the enzyme,but Cu2＋ inhibited it.Conclusion： The cellulase activity was improved 1.4 times compared to original bacteria,meanwhile it has certain practical value to improve fiber degradation during the feed making process.