羰基还原酶产生菌SW 2026的产酶条件及其不对称催化还原4′-氯苯乙酮

Enhancement of asymmetric reduction of 4′-chloroacetophenone by optimizing culture conditions of carbonyl reductase-producing strain Candida krusei SW 2026

以4′-氯苯乙酮为模型底物,对筛选得到的Candida krusei SW 2026的羰基还原酶产酶条件进行研究。结果表明:适宜的发酵培养基组成为甘油50 g/L,玉米浆20 g/L,KH2PO44 g/L,MgSO4.7H2O 1.5 g/L;适宜的培养条件为温度30℃,初始pH6,摇床转速200 r/min,发酵周期48 h。在产酶发酵条件下培养的湿细胞对4′-氯苯乙酮进行不对称还原反应,产物(S)-4′-氯-α-苯乙醇的产率最高达88.56%,e.e.值稳定在87%左右。

The asymmetric reduction of 4＇-chloroacetophenone to its corresponding chiral alcohol in the aqueous phase was chosen as the model reaction to study the effects of fermentation conditions on the asymmetric reduction. Candida krusei SW 2026 showed high enantioselectivity to a series of halogenated aromatic ketones. The optimum fermentation conditions were： glycerol 50 g/L, corn steep liquor 20 g/L, 4 g/L KH2PO4, 1.5 g/L MgSO4 .7H2O, initial pH 6, 30℃, rotation speed 200 r/min, and fermentation time 48 h. After the optimization, the yield of （S） -4＇-chloro-α-phenylethyl alcohol was improved to 88.56% and e. e. was improved to 87%.