响应面设计法优化单葡萄糖醛酸基甘草次酸(GAMG)发酵转化培养基

Optimization of medium composition for glycyrrhetinic acid monoglucuronide(GAMG) production using response surface methodology

以甘草酸(glycyrrhizin,GL)为底物,利用产紫青霉(Penicillium purpurogenum Li-3)液态发酵转化单葡萄糖醛酸甘草次酸(GAMG),采用响应面设计法对初始发酵培养基进行优化。用部分因子分析法研究原始发酵培养基各成分对响应值的显著程度,发现甘草酸(GL)、NaNO3和K2HPO4的质量浓度对发酵产生GAMG的影响显著(P<0.01)。用中心组合设计确立甘草酸、NaNO3和K2HPO4的适宜质量浓度分别为2.8、3.0和0.8 g/L。在优化条件下进行发酵时,GAMG的转化率从75.49%提高到89.11%,比优化前提高了13.62%。

Response surface methodology was used to optimize the initial medium composition for glycyrrhetinic acid monoglucuronide （GAMG） production by Penicillium purpurogenum Li-3 with glycyrrhizin as a substrate. The impact of initial medium constituents on GAMG production was analyzed by fractional factorial design. It showed that glycyrrhizin, sodium nitrate and dibasic potassium phosphate substantially influenced GAMG production （ P 〈 0.01 ）. The optimum concentrations of glycyrrhizin, sodium nitrate and dibasic potassium phosphate were confirmed by central composite design. When the medium composition （ in g/L） was glycyrrhizin 2. 8, sodium nitrate 3.0, and dibasic potassium phosphate 0. 8. The GAMG conversion rate was improved from 75.49% to 89. 11%, under the optimization conditions.