乙型肝炎病毒血清标志物与HBV-DNA定量联合检测的意义

The relationship between HBV DNA and HBV immune Markers in the serum of hepertitis B virus

目的探讨乙型肝炎(下称乙肝)病毒DNA(HBV-DNA)与血清免疫标志物的关系。方法荧光定量聚合酶链反应法(FQ-PCR)检测血清样本中HBV-DNA含量;酶联免疫吸附法(ELISA)检测HBV血清标志物。结果A组(大三阳)、B组(小三阳)、C组〔乙肝表面抗原(HBsAg)、乙肝e抗原(HBeAg)阳性〕、D组(HBsAg、抗-HBc阳性)阳性率分别为97.3%、57.8%、100%、54.3%,HBeAg阳性组和HBeAg阴性组HBV-DNA病毒载量差异有统计学意义(P<0.05)。结论HBV-DNA与乙肝血清标志物乙肝两对半(HBV-M)联合检测,能更准确地反映HBV复制情况,更有助于临床疾病的诊治。

Objective To explore the relationship between HBV-DNA and HBV immune markers in serum. Methods HBV-DNA in the serum were determined by FQ-PCR and serum HBV maekers were tested by ELISA method. Results The level of each group （A,B,C,D）with HBV DNA（＋） were 97.30%,57.8% ,100% ,54.3% re speetively. Meanwhile,there was significant difference between the two groups of HBeAg （＋） and HBeAg （-） of the level of HBV DNA. Conclusion Detection of HBV-DNA and HBV-M can exactly show the reproduction of HBV, and help to diagnose and treat the disease in clinic.