秦艽总苷对人肝癌细胞等几种肿瘤细胞的体外作用

EFFECTS OF GENTIANA TOTAL GLYCOSIDES ON SEVERAL HUMAN TUMOR CELLS IN VITRO

目的研究秦艽总苷对肝癌细胞SMMC-7721、淋巴癌细胞U937、卵巢癌细胞A2780等3种癌细胞的增殖及凋亡的影响。方法MTT法检测秦艽总苷对人肝癌SMMC-7721细胞、淋巴癌细胞U937、卵巢癌细胞A2780增殖的影响,流式细胞仪检测细胞调亡率。结果①秦艽总苷在一定浓度时,SMMC-7721细胞和U937细胞的生长受到不同程度的抑制,且有浓度依赖性;而对A2780细胞无抑制增殖作用。②一定浓度的秦艽总苷可诱导SMMC-7721细胞和U937细胞凋亡;对A2780无诱导凋亡作用。结论①秦艽总苷对人肝癌细胞SMMC-7721和淋巴癌细胞U937有抑制增殖和诱导凋亡的作用;②秦艽总苷对卵巢癌细胞A2780无抑制增殖作用;③秦艽总苷对卵巢癌细胞A2780无诱导细胞凋亡作用。

Objective To study the influence of Gentiana total glycosides on the proliferation and apoptosis of hepatoma cells SMMC-7721,lymphoma cells U937 and the A2780 ovarian cancer cells.Methods The inhibitory effects of tolal glycoside of gentiana macrophylla Pall on cell growth was analyzed by MTT assay,Annexin v-FITC stain with flow cytometry analysis were used to study the apoptosis of SMMC-7721 cells,U937 cells and A2780 cells and the morphologic changes were detected by the method of Wrights-Giemesa staining analysis.Results ① Cell suppression were observed in SMMC-7721 cells and U937 cells while treated with different concentrations of total glycoside of gentiana macrophylla Pall and showed concentration dependent manner.But the proliferation of A2780 cells was not suppressed.②Gentiana total glycosides can induce apoptosis of SMMC-7721 cells and U937,whereas there was no A2780 cells apoptosis induction.Conclusion ①Gentiana total glycosides inhibited proliferation and induced apoptosis of SMMC-7721 cells and U937cells.②Gentiana total glycosides without proliferation inhibiting effect on A2780 cells.③Gentiana total glycosides without the effect of apoptosis induction on A2780 cells.