摘要
本研究以５个春麦品种的幼穗为外植体，接种在ＭＳ为基本培养基不同浓度的激素的诱导培养基上，结果发现，铁春２号是最好的基因型，诱导率、分化率最高，不同品种间诱导率存在极显著差异，６－ＢＡ不利于愈伤组织诱导，最佳生长素配比是２０ｍｇＬ－１２，４－Ｄ＋０５ｍｇＬ－１ＮＡＡ。在分化培养中，最好的激素配比为０２ｍｇＬ－１ＮＡＡ＋０１ｍｇＬ－１Ｋｔ。经长期继代的愈伤组织５℃低温处理１０ｄ，显著提高愈伤组织的分化率。
The explants of the young panicles of 5 spring wheat varieties were cultured in induced MS The result showed that the variety Tiechun 2 was the best genotype and its induction and differentiation frequency were the highest among 5 varieties Difference of induction frequency was significant between wheat varieties 6-BA had negative effect on callus induction. The best combination of growth horomone for callus induction was 2.0 mg.L -1 2,4-D+0 5mg L -1 NAA. The best combination of growth horomone for differentiation was 0 2mg.L -1 NAA+0 1mg.L -1 Kt. Differentiation frequency of callus was significantly high under 5℃ with 10 days in long period subculture.
出处
《沈阳农业大学学报》
CAS
CSCD
1998年第4期297-301,共5页
Journal of Shenyang Agricultural University
关键词
小麦
愈伤组织
出愈率
分化率
Spring wheat
callus induction
differentiation frequency
