p-BAD及p-AKT蛋白在宫颈癌癌变过程中的表达及意义

Expression of p-BAD and p-AKT in Cervical Carcinoma Carcinogenesis Progression and Its Significance

目的探讨p-BAD、p-AKT蛋白在宫颈癌组织中的表达及与临床病理特征的关系。方法用免疫组织化学S-P法检测100例宫颈石蜡包埋组织、Western Blotting法检测30例宫颈癌及癌旁宫颈组织中p-BAD、p-AKT的表达情况。分析两种蛋白在宫颈组织中表达的意义及其与临床病理特征的关系。结果免疫组化结果显示在宫颈癌癌变过程中这两种蛋白表达阳性率呈递增趋势(P值分别为0.001、0.003);p-BAD、p-AKT蛋白在CINⅢ级及原位癌组与宫颈癌组间差异有明显统计学意义(P=0.036、0.048)。两种蛋白与宫颈癌患者的年龄无明显关系,但均与临床分期、组织学分级相关(P值分别为0.037、0.018;0.001、0.002)。p-AKT蛋白的表达与盆腔淋巴结转移有关(P=0.023)。Western Blotting结果显示,宫颈癌组织中两种蛋白含量明显高于癌旁组织(P值分别为0.002、0.003)。结论本试验提示两种蛋白表达水平在一定程度上可能揭示了宫颈上皮内瘤变向宫颈癌的转化的潜能。

Objective To study the expression and significance of p-BAD and p-AKT in cervical carcinoma progression,namely in normal cervical tissue,cervical epithelial dysplasia（CIN I , II and m ）and cervical carcinoma. Methods The expression of the two proteins was ex- amined in 100 paraffin-embedded cervical tissue specimens by using immunohistochemcal staining and 30 freshly-taken cervical carcinoma and adjacent cervical tissue specimens by Western Blotting. The relationship between the expression of the two proteins and clinicopathological factors was analyzed. Results The positive rates of the p-BAD expression and the p-AKT expression showed a gradual increase in cervical cancer progression. The expression of p-BAD and p-AKT proteins showed a significant difference at CIN HI level and also showed a signifi- cant difference between carcinoma in situ group and cervical carcinoma group（P= 0.036,0.048）. No obvious correlation was found between the expression of the two protein and the age of the patients. However,the expression of the two protein had a correlation with clinical stage and histological grade（P = 0.037,0.018; 0.001,0.002, respectively）, and the lower the histological grade was, the higher the positive rate of their ex- pression was. The p-AKT protein expression was related to pelvic lymph node metastasis（P= 0.023 ）. Western Blotting analysis showed that the levels of of the two proteins in cervical eaneer were higher than those in the paraneoplastie tissue（P= 0.002,0.003）. Conclusion The levels of the two proteins could suggest,to some extent,the potential transformation of cervical intraepithelial neoplasia to cervical cancer.