一种基于微流控芯片技术的流式细胞计数系统

Cell counting system with flow cytometry based on microfluidic chip

采用聚二甲基硅氧烷(PDMS)材料制作微流控流式细胞计数芯片,利用负压驱动与鞘液夹流技术实现样品的水力聚焦,达到了10μm的样品聚焦宽度。基于激光诱导荧光技术,制作了小型化的检测装置。以488 nm固体激光器为光源,激光束以45°方向穿过一个水平狭缝,以线光源形式汇聚到微流控芯片的检测区域,并与微通道垂直交叉,细胞样品的荧光信号通过光电倍增管收集。整个分析系统结构简单,操作方便,灵敏度高,可初步实现细胞的计数。

A polydimethylsiloxane （PDMS）microfluidic chip coupled with flow cytometry is developed for cell counting. The sample and sheath liquid are driven by the negative pressure. Sample confinement is accomplished using hydrodynamic focusing by sheath liquid, and the 10p^m of sample width is achieved. A miniature laser- induced fuorescence detector is developed. The 488 nm laser beam passed through the horizontal slit at 45°, and is focused on the microfluidic chip detection region in the formation of line light, vertically crossed with the microchannel,and the fluorescence signal of cells is collected by the photomultiplier. The structure of this analysis system is simple, and it is not only convenient to operate but also has high sensitivity. Initial cell counting can be achieved by this analysis system.