广东虫草多糖的提取及含量测定

Extraction and Contents Determination of Polysaccharide in Cordyceps guangdongensis

采用正交试验方法优化了广东虫草多糖的提取工艺.利用优化的提取工艺对广东虫草子实体、子实体采收后的大米培养基、液体发酵菌丝体以及发酵液4种样品的多糖进行提取,并采用苯酚-硫酸法测定了多糖含量.结果表明,广东虫草多糖的最佳提取工艺为:提取温度90℃、提取时间3 h、提取次数3次、乙醇体积分数100%.子实体、大米培养基、广东虫草菌丝体及发酵液的精多糖含量有所差异,分别为:6.92%、5.40%、6.15%和3.42%.

To get the maximum polysaccharide extraction rate of Cordyceps guangdongensis,the extracting prodedures of polysaccharide were optimized by direct cross experiment.The contents were determined by phenol-sulfuric acid method.The contents of polysaccharide extracted from mycelium,fruitbody,rice medium and submerged fermented liquid of C.guangdongensis were 6.15%,6.92%,5.40%,3.42%,respectively.The optimal extracting conditions of polysaccharide of C.guangdongensis were at 90 ℃,submerged three times with 100% ethanol for 3 h.