猪精子提取物对猪卵母细胞的孤雌激活效果

Effects of Porcine Oocyte Activation Induced by a Cytosolic Sperm Factor

3个试验分别就猪精子提取物提取方法、精子蛋白质量浓度以及显微注射剂量对猪卵母细胞的孤雌激活效果进行了研究.结果表明,采用液氮反复冻融法和超声波破碎法提取的猪精子提取物对猪卵母细胞都有显著的孤雌激活效果;采用猪精子提取物显微注射孤雌激活猪卵母细胞的适宜注射剂量为2-4 pL;猪精子蛋白质量浓度在1.35 mg/mL以上,注射剂量为2-4 pL时均可有效激活猪卵母细胞.

Effects of porcine sperm cytosolic factor （different extraction methods, different albumen concentrations and different micro-injection doses ） on parthenogenetic activation （PA） of porcine oocytes were investigated in 3 experiments. The results showed that there was a significant activation effect of oocytes after injection of cytosolic sperm factor extracted either by repeated frozen-thawed or ultrasonic fragmentation method. The optimal micro-injection dose for PA was 2 -4 pL per oocyte. Porcine oocytes could be effectively activated by micro-injection of 2 -4 pL porcine sperm extract, in which albumen concentration was above 1.35 mg/mL.