胰岛素和半胱氨酸对猪孤雌胚早期体外发育的影响

Effects of Insulin and Cysteine on the in vitro Development of Porcine Parthenogenetic Embryos

探讨在NCSU-23中添加不同浓度的胰岛素和/或半胱氨酸对猪孤雌胚体外发育的影响.结果表明,在试验1中,添加胰岛素的质量浓度为2.5μg/mL时,猪孤雌胚的卵裂率及囊胚率显著高于对照组(P<0.05);在试验2中,添加半胱氨酸的浓度为1.2 mmol/L时,猪孤雌胚囊胚发育率最高,但添加的浓度大于4.8 mmol/L时,猪孤雌胚的囊胚发育率显著降低(P<0.05);在试验3中,联合添加胰岛素和半胱氨酸时能显著提高猪孤雌胚的囊胚率(P<0.05).因此,在NCSU-23中添加一定浓度的胰岛素和/或半胱氨酸能提高猪孤雌胚发育到囊胚阶段的能力.

This study was to evaluate the effects of insulin and cysteine on the in vitro development of porcine parthenogenetic embryos. After parthenogenetic activation, embryos were transferred to culture medium（NCSU-23） supplemented with different concentrations of insulin and/or cysteine. In Experiment 1, when the concentration of insulin in the culture medium was 2.5 μg/mL, the rates of cleavage and blastocyst were significantly higher than the control group（P 〈0. 05 ）. In Experiment 2 ,when the supplementation concentration of cysteine was 1.2 mmol/L, the blastocyst rate of parthenogentic embryos was the highest. In Experiment 3, a combination of supplementation with 2.5 μg/mL insulin and 1.2 mmol/L cysteine in the culture medium could significantly increase the blastocyst development rate （ P 〈 0. 05 ）. It is concluded that supplementation of a certain concentration of insulin and/or cysteine in the culture medium can promote the development of porcine parthenogenetic embryos in vitro.