人参养荣丸中人参皂苷R_e的含量分析

Determination of Ginsenoside Re in Renshen Yangrong Pills

目的分析人参养荣丸中人参皂苷Re的含量,为其质量控制提供依据。方法色谱柱为Shim-packCLC-ODS色谱柱(250mm×4.6mm,5μm),保护柱为DIKMAEasyGuardC18柱(10mm×4.6mm),流动相为乙腈-0.5%磷酸(21:79),柱温为26℃,检测波长为203nm,流速为1.00mL/min。结果标准曲线方程为C=3.50786×10-7A+0.00054,r=0.9998,(n=6),线性范围在0.012～0.24mg/mL,平均回收率为101.26%,RSD=4.18%(n=6)。结论该方法前处理简单,分析准确、快速,可作为人参养荣丸中人参皂苷Re的含量分析方法。

Objective To determine quantitatively ginsenoside Re in Renshen Yangrong Pills, and to provide the experimental evident for its quality control. Methods The method was developed with the condition as follows： Shim-pack CLC-ODS column（250 mm ×4.6 mm, 5 μm）, Dikma Easyguard C18（10 mm×4.6 mm） as the protection column. Elution was employed with the mobile phase of acetonitrile-0.5% phosphoric acid（21：79）at the flow rate of 1.00 mL/min. The column temperature： 26 ℃. The detection wavelength： 203 nm. Results The standard curve equation： C =3. 507 86 ×10^-7A ＋0. 000 54, the linear range： 0. 012 -0.24 mg/mL, r =0. 999 8, （ n =6）, the average recovery rate of the added sample： 101.26%, RSD=4. 18% （n =6）. Conclusion With its accuracy, sensitivity, reproducibility as well as the simplicity of sample pretreatment, the method is feasible for the quality control of Renshen Yangrong Pills.