高效液相色谱法测定麻杏定喘合剂中苦杏仁苷含量

Content Determination of Amygdalin in Maxing Dingchuan Mixture by HPLC

目的探讨测定麻杏定喘合剂中苦杏仁苷含量的方法。方法采用高效液相色谱(HPLC)法,色谱柱为Dikma Kromasil C18柱(250mm×4.6mm,5μm),流动相为水-甲醇-乙腈(75:20:5),检测波长为210nm,流速为0.8mL/min,柱温30℃,进样量10μL。结果苦杏仁苷的平均加样回收率为99.02%,RSD=1.37%(n=6),平均含量为1.3575mg/g。结论所建立的方法操作简便、结果准确,可作为麻杏定喘合剂中苦杏仁苷的含量测定方法。

Objective To study the method of amygdalin determination in Maxing Dingchuan Mixture. Methods HPLC was adopted with Dikma Kromasil C18 column（250 mm × 4.6 mm, 5 μm）and the mobile phase was water -methanol -acetonitrile （70 ： 20 ： 5）. The detection wavelength was 210 nm. The flow rate was 0.8 mL/min with the temperature of 30 ℃. The sample volume was 10 μL. Results The average recovery rate of amygdalin was 99.02% with RSD of 1.37% （n =6）and the average content was 1. 357 5 mg/g. Conclusion Besides simplicity, the method yields accurate and reproducible results, which can therefore be used in the quality control of amygdalin content in Maxing Dingchuan Mixture.