低温弱光条件下砧穗互作对茄子嫁接苗抗冷性的影响

Effect of Interaction Between Rootstock and Scion on Chilling Tolerance of Grafted Eggplant Seedlings Under Low Temperature and Light Conditions

【目的】探讨茄子砧木及接穗对嫁接苗抗冷性的贡献大小,为茄子越冬嫁接栽培合理选用品种提供参考。【方法】以抗冷性显著不同的台茄(T)和赤茄(C)互为砧穗,采用靠接法进行组合嫁接,测定低温(10℃/2℃)胁迫前后嫁接苗叶片电解质渗漏率、丙二醛(MDA)含量及主要渗透调节物质含量的变化。【结果】低温胁迫下,TC/TC、TC/C、TC/T等组合嫁接苗中台茄叶片的的电解质渗透率及MDA含量均显著高于同株的赤茄叶片,但显著低于T/T,且组合嫁接苗中台茄叶片的可溶性糖、可溶性蛋白和脯氨酸含量较T/T显著升高,表明抗冷性较强的赤茄根系及叶片,均对嫁接苗台茄接穗抗冷性具有正向效应。虽然TC/C的抗冷性较强,TC/TC次之,TC/T较差,但三者均显著低于C/C,表明抗冷性较弱的台茄无论根系还是叶片,均对嫁接苗赤茄接穗抗冷性具有负向效应。【结论】由于TC/C的抗冷性显著高于TC/T,表明嫁接苗抗冷性以根系的贡献大于接穗。

【Objective】 In order to provide a reference for selection of reasonable species for eggplant grafted during winter planting,the contribution of root and scion to cold resistance of grafted eggplant seedlings was studied. 【Method】 Two rootstock eggplant cultivars,Daidaro （T）,susceptible to cold and Hiranasu （C） is more resistant,were used to be grafted on each other. Based on this strategy,the physiology parameters including electrolyte leakage,MDA content and main osmotic adjustment substances in grafted seedling leaves before and after low temperature stress were studied. 【Result】 After low temperature treatment electrolyte leakage and MDA content in T-leaves of TC/TC,TC/C,TC/T was significantly higher than C-leaf in the same combination,but were observably lower than that of T/T,meanwhile,the content of soluble sugar,soluble protein and proline in T-leaves of combination of grafted seedlings was notably higher than that of T/T. All the results above indicate that both root and shoot of Hiranasu can enhance cold resistance of Daidaro in grafting experiment. Although TC/C had more resistance than TC/TC and TC/T which is the most sensitive to cold,all three of them were in the shade compared to C/C. So it can be concluded that both root and shoot of Daidaro had a negative effect on cold resistance of scion Hiranasu. 【Conclusion】 TC/C was more cold resistance than TC/T,so root had more contribution than shoot to cold resistance of grafted seedlings.