相干反斯托克斯拉曼散射显微成像技术

Coherent Anti-Stokes Raman Scattering Microscopic Imaging Technique

回顾了相干反斯托克斯拉曼散射(CARS)显微成像技术的理论和技术的发展,介绍和比较了CARS显微成像技术对抽运光源的要求,以及典型的CARS显微成像系统。对CARS显微成像技术中无法避免的最重要的非共振背景噪声问题做了详细的分析,对不同的抑制非共振背景噪声的方法进行了比较和讨论,对CARS显微成像技术目前存在的问题和可能解决途径进行了简要的分析。

In this paper,theoretical and technical development of coherent anti-Stokes Raman scattering （CARS） microscopy is reviewed. The requirements of CARS microscopy on the pump laser source and typical experimental instrumentation are discussed and compared. We investigate the non-resonance background noise which is the most critical problem and can not be avoided in the CARS microscopy. Different methods for suppressing non-resonant background noise are presented and compared. Finally,a brief analysis of existing problems in CARS microscopy and possible solutions is presented.