动脉粥样硬化斑块和血管壁的光谱学实验研究

Experimental Study on Spectroscopy of Atherosclerotic Plaque and Vessel Wall

为区分动脉粥样硬化斑块和血管壁,给激光血管成形术的实时监控提供依据,研究了动脉粥样硬化斑块和血管壁的激光诱导荧光光谱(LIFS)和拉曼光谱。日本雄性大耳白兔15只,以高脂饲料喂养3个月,取主动脉弓作纵行切开,分别采用380 nm紫外激光和532 nm绿激光作为激发光源诱导动脉粥样硬化斑块和血管壁的荧光光谱;采用532 nm绿激光作为激发光源诱导动脉粥样硬化斑块和血管壁的拉曼光谱。结果表明,380 nm紫外激光诱导的动脉粥样硬化斑块和血管壁的荧光光谱在416 nm处均有明显特征峰,但前者的强度显著高于后者;532 nm绿激光诱导的动脉粥样硬化斑块和血管壁的荧光光谱在800 nm处均有明显特征峰,但强度无明显差别;动脉粥样硬化斑块的拉曼光谱在3000 nm和3300 nm处存在明显的波峰和波谷,而血管壁的拉曼光谱曲线较光滑,无明显特征峰。说明380 nm紫外激光诱导的荧光光谱和拉曼光谱均有可能有效区分动脉粥样硬化斑块和血管壁,而532 nm绿激光诱导的荧光光谱不能有效区分动脉粥样硬化斑块和血管壁。

The atherosclerotic plaque and vessel wall are experimental studied by laser-induced fluorescence spectroscopy （LIFS） and Roman spectroscopy in order to provide a real-time monitoring for laser angioplasty. Fifteen male Japanese white rabbits fed three months with high-fat feed get aortic arch and do portrait incision by using 380 nm ultraviolet （UV） laser and 532 nm green laser as light source to induce fluorescence spectroscopy of atherosclerotic plaque and vessel wall respectively, while 532 nm green laser is used as light source to induce Raman spectroscopy. Results shows that, 380 nm UV laser-induced fluorescence spectra of atherosclerotic plaque and vessel wall both have characteristic peaks at wavelength of 416 nm, but the intensity of the former is significantly higher than the latter; 532 nm green laser-induced fluorescence spectra both have characteristic peaks at wavelength of 800 nm, but have no significant difference in intensity; The Raman spectroscopy of atherosclerotic plaque has one peak at wavelength of 3000 nm and one trough at 3300 nm, but the Raman spectroscopy of vessel wall is smooth without any peaks. Therefore, both 380 nm UV laser-induced fluorescence spectroscopy and Raman spectroscopy can effectively differentiate atherosclerotic plaque and vessel wall, while 532 nm green laser-induced fluorescence spectra cannot differentiate them effectively.