摘要
为研究促卵泡素(FSH)在奶牛卵母细胞的成熟过程中的促进作用,在牛卵母细胞成熟液中添加0μg/mL、10μg/mL、25μg/mL、50μg/mL、100μg/mL FSH统计成熟率。结果表明,添加FSH各组与对照组相比,卵母细胞的成熟率均显著提高。而添加10μg/mL、25μg/mL、100μg/mL组卵母细胞的成熟率之间差异不显著,50μg/mL组卵母细胞的成熟率最高。无论是否添加FSH都不会影响到孤雌激活的卵裂率。说明FSH具有促进奶牛卵母细胞成熟的功能。
To observe the influence of Follicle-stimulating hormone （FSH） on in vitro maturation of bovine oocytes,0 μg/mL, 10 pμg/mL,25 μg/mL,50 /μg/mL, 100 /μg/mL FSH were supplemented in oocyte maturation media and the rate of maturation was counted. The results shows that the presence of EGF during in vitro maturation （IVM） significantly increased the proportion of oocytes. FSH with 10μg/mL, 25μg/mL,100μg/mL have no significant influence on promoting oocyte maturation in vitro, while FSH with 50μg/mL the best . However,the cleavage rate was not affected by FSH,revealing FSH can promote the activation of oocytes.
出处
《家畜生态学报》
2009年第5期29-30,35,共3页
Journal of Domestic Animal Ecology
