基质金属蛋白酶2和9在膀胱移行细胞癌中的表达及其意义

Correlation of expressions of MMP-2 and MMP-9 in the transitional cell carcinoma of bladder with tumor invasion and metastasis

目的探讨膀胱肿瘤组织中基质蛋白酶-2（MMP-2）及基质蛋白酶-9（MMP-9）的表达与膀胱移行细胞癌（TCCB）侵袭转移的关系。方法选择新乡医学院第一附属医院2006年8月至2008年8月间行TCCB切除术后患者46例作为试验组，并取14例癌旁正常组织（肿瘤周围3～5cm）作为对照组。采用免疫组织化学（EliVision法）检测MMP-2及MMP-9在膀胱肿瘤组织中的表、达。结果MMP-2和MMP-9在TCCB中的表达显著高于癌旁正常组织（P〈0．01），Ⅱ、Ⅲ级TCCB中的阳性表达明显高于I级（P〈0．01）；浸润性及Ⅲ级TCCB组织中的阳性表达高于浅表性及Ⅱ级中的表达，但差异无统计学意义（P〉0．05）。结论MMP-2及MMP-9的阳性表达与TCCB的恶性程度及侵袭转移有关，可作为判断膀胱肿瘤预后的有用指标。

Objective To evaluate the expression of MMP-2 and MMP-9 and their relation with tumor invasion and metastasis in transitional cell carcinoma of bladder （TCCB）. Methods Immunohistochemistry of EliVision were respectively used to determine the MMP-2 and MMP-9 expression in tumor tissues of 46 patients with TCCB and 14 cases of control group which were screened quantitatively （3-5 cm of surrounding tumor） between August 2006 and August 2008. Results Expression of MMP-2 and MMP-9 in TCCB are obviously higher than the normal control group （P 〈0.01）, expressions of MMP-2 and MMP-9 in level Ⅱ, m are obviously higher than in level Ⅰ （P 〈0.01）, but there was no significant relationship between high stage or level Ⅲ and low stage or level Ⅱ （P 〉0.05）. Conclusion The positive expressions of MMP-2 and MMP-9 in tumor tissues correlated with the malignant degree, invasion and metastasis of TCCB, MMP-2, MMP-9 could be useful indicators for the assessment of prognosis of TCCB.